FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTACTCTTTGTTCATTTGAATTGACATCTCAAGTGTTCATTGCTTTAA22.5316455696202533No Hit
ATATATATATAGAGAGAGAGATAAGTAATTCGAAAACATATAAAAGAAAT22.5316455696202533No Hit
ATACCTTCAACAATTATTATTATCCATGTGAGTCTTTAACTTTTGTTTGT11.2658227848101267No Hit
CAACAATAAAAAAAAACAAACATGAAAATAATAAAATAGAAAAAAAAAAT11.2658227848101267No Hit
CATATATATTATAATAAACCACCAAATCCATATTTAAAAAATTTGTAAAA11.2658227848101267No Hit
CTCTCATGCTTTAAGCAAACTTGTATACAGCATTTTTCTGACCCTTTTCT11.2658227848101267No Hit
GAAGTTTCTTTTTTCTTTTTCATGGCGATTGCTTCCGCCGCTGGTAAAAA11.2658227848101267No Hit
GAGTATAGAAAGGCCCGAGGCGCCGTGAACCGTAAAGGTGTAGAATTGGA11.2658227848101267No Hit
GCTGCAACACAAATATAAATGCTCCTAATCGAGATCAAGGAACTCCATTC11.2658227848101267No Hit
GGTTGAGAAAAATAAAAAGNTAAGAGTGCCAAATTAGAGTCTGTTGGTAG11.2658227848101267No Hit
GTGCCCTGAGTGGCGGAGCAGTATACCGGCATTCAAATGCAAGGGGTGCA11.2658227848101267No Hit
CTTTAGATGAATGTAGGGGCATGGGCCATGCTTTGACGTGGGCCTGATGT11.2658227848101267No Hit
CATTAGGATCCCGATTTATGGATCTCGCGGTTCGAGAAAGCAAAATAAGA11.2658227848101267No Hit
ATTTAAAGAAGCGGTGTTGGATTATGCTTCTAAAACTAGTCGCAACATTA11.2658227848101267No Hit
CTCTTAAAAATGCTTATAAATAAATGCAAGACAGGGGATTCCTTTGTGTC11.2658227848101267No Hit
TAATCTATCCATGTTGATCGACACACATCTGGTGTCGGTCAACGTGAGTG11.2658227848101267No Hit
GGTCTCCGATGACTGAAGAAGAAGAAGGACAGAGCCACCAATCGCCAGTT11.2658227848101267No Hit
CTCTTCTCAGTCGCCTCGATCTTCGTCACCGGAAATTCCATCCCTCCGAC11.2658227848101267No Hit
ATACAACAATGTGCAATTAAGATCAGGCACATATAGAACATTAAGCAGAG11.2658227848101267No Hit
GATCTAGATTCTATAAATAATTAATATAATAAGTCATAACTAATAAAATA11.2658227848101267No Hit
ATTCTATTGGGTCTGACTCGTAGTGATCATTTATCAAAGAATGACTCTGG11.2658227848101267No Hit
GTTTAAAATATTTTACTCGTACTCTTTTTGCCAAATCTCTGGGAATCTAT11.2658227848101267No Hit
ATTAAATACTTAATACCATGTTTGGGATAATGGCCATGTTTAAAGTATTG11.2658227848101267No Hit
GTGATACGATTAGGTCTCATCCGATCTAAAAAATCTTATTTTTNTGAATA11.2658227848101267No Hit
AGTTGATTGATACTATATTTCTTTCTTGTTGTATCTTGAGTGTCTGTACT11.2658227848101267No Hit
GAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGCCGTTTAGGC11.2658227848101267No Hit
AGATTAAATATTGCCATTTCTTATCAGGAATTTCATTTGAAGACTTCAGA11.2658227848101267No Hit
AAGATATCCCAAGAGAACTCATAATCTAGATGAAAATCGGATCTAACAAC11.2658227848101267No Hit
GTATATCACATAGTCTCCTTACTATTTCCTCCCATGAGGTTGTACTGCAT11.2658227848101267No Hit
GAGTTAAATTTTGCAATTACCTGACCACGAAAAGGTACTTCTCTTTCAAT11.2658227848101267No Hit
GTTTTAGTGTCTTTTCAGGTTTTCACTTCCCTGGAGCTAAAATGTGCAAA11.2658227848101267No Hit
CTTCTAGACAATAGCCTATTGCCTTTCTAAATTTTCGCAATTCGCTGAGC11.2658227848101267No Hit
CTGTTTGTGGCATATCCAAGGAAAACCCCTTCATCAATTCGATAATCAAA11.2658227848101267No Hit
GTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTT11.2658227848101267No Hit
AGTTCATGCTCTTCCCTTTGCTCAATCGTGTGTACTACCCAGGCTCATTG11.2658227848101267No Hit
GACTGTGACTTTGAAGTAGAAACAGGTAATGAAAAATGGGAAACAATAGT11.2658227848101267No Hit
TTTCCAGTCTTTAAATCGAACCATATAATCTAGGGTCTTTATAATTAAAG11.2658227848101267No Hit
ATAATGTCCCTTACACTGATGCAAGACGTAATGGAAAGGTTAAAAAGATT11.2658227848101267No Hit
CAATGGGCTAGGGTGGCCCCATTTCGCCTTACCAACCCCAGGGAAACTCC11.2658227848101267No Hit
CTCAAACTCCTGTTCATATTTAATGTATACACACCAAACTTTACCATAAG11.2658227848101267No Hit
GATTATAAGTTCATAACCTTAGTCCATTCCCATTTTGGGCGGAACCGATC11.2658227848101267No Hit
ACCATTGACTGTATCTGCAGCTGCCAGTATCAACTGTCCATGGCAAATAG11.2658227848101267No Hit
AGTTTGTGTAAGTATGGAATCATGAATAGTCATAGTTCAGTCAGTATATC11.2658227848101267No Hit
ATTCAGGAAAGCAAAATCAACATTGGAGAAAGAAGCATATTCTGCATAAT11.2658227848101267No Hit
GTAATAAGATATCGGCTACAATTGTAAAGGTTTTATCAATAAAATTTCCA11.2658227848101267No Hit
ATATTGGAAAGTATCGTTATAAGAACAGCTTTGCTGAAACTGGAGCTAGC11.2658227848101267No Hit
CATAAGTCCCTCCCTACAAGTCATGAATTTAGAATTCTTGCAATAAAACA11.2658227848101267No Hit
ATATTGACTTGTCAAAAACCTCTACACCATAGAGCCTGTAGAGTATGCAG11.2658227848101267No Hit
GTAACGTGACATAAAGACTCCTTTTTTTATTGAAATTGTACAAAACTAAA11.2658227848101267No Hit
GTTTTCCTGAGCGGCCAGAGAGACCTAGACACGATTTTTCACAGTTACTC11.2658227848101267No Hit
CAAGGTTACTTTCTTTCTTGATGTGAGCCGTTGAAAGATACTTTATGTAA11.2658227848101267No Hit
TTACCCAGGTCCGTTTCTATCTCTTCATTTGTGAATTTTTCAATTTTGTG11.2658227848101267No Hit
CCCCGTTATAAATTCTATCCATATAGATTCTGATCGCCAACTCATATATA11.2658227848101267No Hit
TCATCATCATGTTATTTATAATTTTAACAAATATATATTGATGATTGAAT11.2658227848101267No Hit
ACATAATACATATCCGAAAGGAAAGGTTAAATCACTTCCGTTGAATCAAA11.2658227848101267No Hit
CTTGGAAAGCCAGTCTACATGAGATGTGAACAACCTTGAGCAAATGTAAT11.2658227848101267No Hit
AAACTGCACCAATAGTCAACTCAAACTAATATAGATGCTGTCTCTTATAC11.2658227848101267No Hit
AAACAACACAAAAACAAAATACAAAATCACTATATATATAAATATGTAAC11.2658227848101267No Hit
GTATATAATATAAGTTTTGACGGCGTGTGAAGTATATACAAGCTTTTTTA11.2658227848101267No Hit
AAATAATAGAATATATCACAAATATAAAATAAATAAACAATTAAAAAAAA11.2658227848101267No Hit
CTAGAGAACAATACTTATCAAATAGAAAATGAAATATGACAAATAATCCA11.2658227848101267No Hit
ATGCCGGAACCGACGCGAACCACCTATCAACACACAAGAAAAGCAAGAAA11.2658227848101267No Hit
TCAGGAAGTACCGTTTGTGGAACCTCAATATCCACGGGCGTATTAGCTAA11.2658227848101267No Hit
CTTTCTACCATTTGCACTTCCAAAAGACATTTGGGAGTTTTGGAGCGAAA11.2658227848101267No Hit
GTTTTATAGAGATTCCGACGATAATGGGAGTCAATCTGATTAACATGAGT11.2658227848101267No Hit
CATAAGAATGGCACGAGTGATTATTTCTTTTCCCATAGTGGCATAAGAGG11.2658227848101267No Hit
GGAATATACAGAATTGTTATCAATCAAATATTGCAAAGTCCTGGTATCTA11.2658227848101267No Hit
GCCTCTCCCTCCGTTACTCCGCCTCTTTCTCTTTTTCATGTGCAGGGGAT11.2658227848101267No Hit
ACATTCAATCGAACAGCCAGCGTTCGTCGCCCCGGAGACGGTGCGTGTGC11.2658227848101267No Hit
CAAATAGAGATTTTGTTTCGTTGGCTCTGTCTTCGGCTCTTCAAGACCCA11.2658227848101267No Hit
CAAAAATGAGGCTCACCATAGAGATTTTCTATGTCCTCCGAGAAGACACA11.2658227848101267No Hit
GCTTAATAGGGGTATCGGTTACCGTGGACCGGAAATCTTGAATCAGTGAA11.2658227848101267No Hit
ACAATGGGACGATAATACGATATGATGACAATGCCGCAGTTGTCATTGAT11.2658227848101267No Hit
GAAATGTACTCACAATCTTCTCTTCCTTTTCAGCTTTATTGAGCGCTTCT11.2658227848101267No Hit
GAATAGAAGAGCTTGCCCCACCCATGAGTAAATATTTCATAGTAGCCTCA11.2658227848101267No Hit
CCTCTGTGGTATTATCATCTCGTTCCCACACCCATTGCTCACCATTGTTG11.2658227848101267No Hit
TAACAGGTAGTGCTGAACTTTTTCTTTCTTTAGCGTTTGCTACAATTTAA11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)