FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATGATTCAGAGTATCATTTCCTATTAGATCACTTTGAATTCCATA17.6923076923076925No Hit
CTGAAGCTTCGAGGCCTCAAGTATCACCGAACTCTTCCTCTCAGTATCAA17.6923076923076925No Hit
AAAAAAAAATCAAAAAAAAATTACGAGAATTAAGAGTCCNTAAAAATAAA17.6923076923076925No Hit
AAGTTCCTCACACCAAAATAAAGAAATAGTTAATGATACAATCATCCAAT17.6923076923076925No Hit
GTTTTCTATTGACACAATTTAGTTCTTTTTGAACATTCATCTGTAATTCT17.6923076923076925No Hit
CATCAGGGACTCCCAAGCAAACAAATTCTCTAAAAACTATAAGTAGATAA17.6923076923076925No Hit
GCTTTCATATGAGTCGATATTTCAGATACTTAGGACTGGTTCTGGATCAT17.6923076923076925No Hit
CAATATCTCCTAGTTCGTAAGTAGAATGTTCTGATGAAGAAGACTCCTTA17.6923076923076925No Hit
GGAGAAATGAGTCAGGAAGTGATTAAGAAGGTGATTAAAGCCTTAGCGAT17.6923076923076925No Hit
AAAAAAATAATTACAATAATAAATAAAGAACTAACAATAAAAAATACTAC17.6923076923076925No Hit
CAAGGATATATGTGCATATGCTGATGATGTGTATAGCACTGCTACATGGA17.6923076923076925No Hit
GATTAGTAATCCAAACTTGATAGAGAATGTTAAACCGGCTAATGGTAATT17.6923076923076925No Hit
CATTGAAGTTTCCTTTATCTATCCTTTATTTGTGAATCCCAAGTGGGAGT17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)