FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATCGATGTAGCTAGGTTTTAAGTAAAAAATGAAAAAGACAAATAAAG24.3478260869565215No Hit
ATATAACTTGCAGCTGTATCACTCAAAACTCACAAACATCTGATGTATTT24.3478260869565215No Hit
GAGAAACAAGGAGAAAGATATGCTAAATTTGTTTTAATTGTAATTTTTTT24.3478260869565215No Hit
GAATACATTCGTCACACGTGAATCATTCACAATCATGGTTTATTTTGCAG24.3478260869565215No Hit
ATATTTAGACAATATAGGCTTTTCTCCTTCATCCTTTTTGATTTGAAGTT24.3478260869565215No Hit
CTTCTTATGGATTGATTGAGAATGATTCGGATCTAGTTCATGGCCTATTA12.1739130434782608No Hit
CCTGAAAGCACTTCTGTTTCTTTCACTTAAAGATAGCGTCTCTTCGTCTT12.1739130434782608No Hit
GGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCA12.1739130434782608No Hit
GTTTTGAAGACAATTTTACCTCTTAACAATAATGTGATGAAAACTTTTGA12.1739130434782608No Hit
GGTAGGATAGTGGCCTACCATGGTGTTAACGGGTGACGGAGAATTAGGGT12.1739130434782608No Hit
GTAATTTACAACGACAAGAAGATAAGTGTGGAATCAATCGAGGTAGAAGA12.1739130434782608No Hit
ATTCTCAAGAAATCTCGGTTAGAGATCGCCGATGCTGGAGTTGATGATAA12.1739130434782608No Hit
ATATATATATATATATATGAGATCAAATCTATATCTTAGTTAATATTAAA12.1739130434782608No Hit
CTCACTCACCAGATTCATCACTTGGGTCCGAAACCCAAAAATCTCTAAAA12.1739130434782608No Hit
CTCATAAACCATAACTGTTCTTAGGGATAAAGGTCCAATTCCACTTATGT12.1739130434782608No Hit
CTCTAGACTCTAGAATGTAATAATTAACTTTTAGTAAAGTCTCATCAATG12.1739130434782608No Hit
GGCTAAGCTCCATACAGTTAGAGTGATTTTATCATTGGCAACCAACCTAT12.1739130434782608No Hit
GAAGATAAGTTACCTTGTCCAAGAAGGCAACAAAGAATGTCAAGACTATA12.1739130434782608No Hit
GGGAGGTAGTGAGAGAATGAATGTGAGAGATTAGAGAAATGGAGAGTGAG12.1739130434782608No Hit
GAGCTAATCCTTCGGATTTCACTTTTGATGGACGTAGTGCTGTTAATATA12.1739130434782608No Hit
GATTGATACAATCTAAACCTCTGGTAAAAAGCCCCCCGTAACCCAGCAGA12.1739130434782608No Hit
GGGGTAAGTAGTACATGAATATGAGAATATCTATATATTTAGTTTTTTCT12.1739130434782608No Hit
CATATGTATGCTCAAATTTGTATTGGTGTTGGACTTATTTTAGTCTCAAA12.1739130434782608No Hit
ATTACATGCGTTAAGAGAAATTAACTTACTCTCAACAACAGTAAGGGCTT12.1739130434782608No Hit
GGTGGTGGTGGAGGTGGTGCGTTTGAATCGAGCCCATATATGATGCAGCA12.1739130434782608No Hit
AGTGTTTCTACTTATCATTCCAAAATTTCCGCAAGAAAGATATTTTGTGA12.1739130434782608No Hit
ATGGAGAAGGGTGGGTACTTGGTAGGTGCTATTTTAGAATTATATGGAGA12.1739130434782608No Hit
ACTATGACCATGAAAAGNATGCTTCTCGATGCAATTTAAAGTTTTATTTG12.1739130434782608No Hit
GAACCTAAACCTGCGCTCGGGAGATAGCTGTCCATACACTGATCAGGGAT12.1739130434782608No Hit
AACTCAATGTGTTCCATTGGCATATCACTGATTCACAATCCTTTCCGTTG12.1739130434782608No Hit
CCAAGTTCTTTCTTTAATCTTCCCAATAAGTTATTATGCTTCTAAAAAAT12.1739130434782608No Hit
TCTAACCCCAGAGGAATCTCCAGAATTGGGCCGCCCTGGATGGTTCTACA12.1739130434782608No Hit
GTATTCAAGCTTTTTAAGTCTACAATCTTCCCTAGATTTGGAGTGCCGCA12.1739130434782608No Hit
ATCATATGTTAAATTTACAGATAGTTACCAAATAAATCTACCACCGTGGC12.1739130434782608No Hit
TGTCAATACTGACAACAATGAAATTTATAGTAAGATGAAAATCCGTTGAC12.1739130434782608No Hit
ATCAGATATCTCAAACATAGTCCCGGACATGATATTTACTTAGAGCGGAT12.1739130434782608No Hit
GTCGACACACACACACCATCACAAGTCACGTTTTTCAAAATATAACAGTT12.1739130434782608No Hit
ACTCCAAGAGCACTCATCCATAAACCAGTTACCGGTACAAATAACATAAA12.1739130434782608No Hit
TATATATATAGGTTCCCGATGTGTCGCCTTTCGAAATCAAGATCTTGGGA12.1739130434782608No Hit
GTTACCTATCACTCAAAAAGCTCTATCTCAAGAGCAAATTGGGAAGCTTC12.1739130434782608No Hit
ATATTACGATTAAATATAATTAATATTTTGGATATTAAATTAATGTTTGG12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)