FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATTTATTAGATCGTGTCCTGAGGCATCGCGATGCAATCATATCACAT27.142857142857142No Hit
GGTGTTGTGTTTGTTTGAATCTCTATCGTTACTGCAGTCTGAGTTTGTGT13.571428571428571No Hit
AATCAAGCCCCAAGACAAGTTGGTGTACTTCTGGGTAAGCCCGAGGAGAA13.571428571428571No Hit
CATATGAGGTTCTCANGTATTTTGACACTAGCAGAGAAGCATGGATCAAC13.571428571428571No Hit
GATCAAACTTAGTCTCTCGGACTTCTAAAACATAATTTCACCTAAAAACA13.571428571428571No Hit
CTTTTTGGTAACCTTAAGAAATGCACTTTTTGCACAGATAACCTTGTGAT13.571428571428571No Hit
GCCTCCGTCACCAAAGTCACCAATGTCGCCAAAGTCTCCGTCATCCTGCA13.571428571428571No Hit
AGAAAGGATTGTGGAACAGGGTCCTTCGTAGCAAGGAAAGTCCTAGCCTT13.571428571428571No Hit
ATATCAATAACGAAAAAAATAAAAGTATATGATTAGATCAATGGTCTGAA13.571428571428571No Hit
TACTTGTCTCGAGGCTTTCCATACCATCAAAGGAGCCCTTGGGAGGGCCC13.571428571428571No Hit
TCTCTCCAATATCGAGTAGTCTTTGAAGATCTTCTGGTGTGGGTCTTCTT13.571428571428571No Hit
ACTTTAGAAACNGATAGAGAAAGGATTTGAAGCTGTTCTGCAAGATGAGC13.571428571428571No Hit
TTCCTTTTCCGTTTCGACTCTCTTTGCTCCGGTTCAATTAATCAACCTAC13.571428571428571No Hit
ATCAAAGCTAATTCATGGTTTTTTCTAATCAATTTCAGGCTGCAGTCTGA13.571428571428571No Hit
AGTGGTGAATCTGAGTATAAAAAATGGGAACAATTATCTGTCGTCCGAAT13.571428571428571No Hit
CCCTCCTTTATTGTGACTGTTCACACTTGGGACAGTGTGATTCAAGTCTG13.571428571428571No Hit
GGACTGTGTAGAATAGCCTGGAAAATCACTTTGAAACATATTTGCAGAGT13.571428571428571No Hit
TATCTATAGTACTATTCCCAGCTCACATGAAGCTCCTTTTGTTCACTTGA13.571428571428571No Hit
ATAGCATACTCCTTCTCAATCTCAGCCTTGAACCATTCTGGGAAAGGACT13.571428571428571No Hit
ATCTTCTCTCTGTGGGATCAACATACCTTAAGTACTACGAGTGATTGCTG13.571428571428571No Hit
TCTTCTTGCTGAACATGCTGCTGCTGCTACTGGTTATTGTAGCGAAAAGG13.571428571428571No Hit
ATTTAAAACTTATATCTTGCAATCTTGCATTATCGTTGGGGAAAACAAAG13.571428571428571No Hit
CTTATATAATTCTCATGTATGTGAATACGAATCCATCTTTTTTTTTCTAC13.571428571428571No Hit
TCACCATATACTAAACACTCACTAATGAGTACCGCATCTTCAAAATTGTA13.571428571428571No Hit
GATTTCTTCAACGAATACATAAAGGTTGGAGCAGCACAGAAGAAATCGTT13.571428571428571No Hit
GCTTTGTAATTCACCATATCTGCATACCATGGGACACCACCTTCCACCAA13.571428571428571No Hit
TACTTAGATGGGAACTTCTTGACGGGAAAACCTCCGGCAAGGTTTGTGAN13.571428571428571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)