FastQCFastQC Report
Tue 23 Aug 2022
HNCMMDSX3_n01_ath_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCMMDSX3_n01_ath_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACATACCAGGTATTATCCCGTACACATGTTGAACNCAGTTGTACA34.225352112676056No Hit
GAGTGGTAGCTGATACTCTATCCCTCTGACTCTGAAGCTCACATGCCCCA22.8169014084507045No Hit
ATTAGATATTTAATTCTCAAAGAAAAGTTGCATTTAGAATTCAAATTTCG22.8169014084507045No Hit
GCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGG22.8169014084507045No Hit
GGACCCTATGCGGTAGCTCCACCACTGCCACCGGCTCAGCAGACATTGTT22.8169014084507045No Hit
CTGTATCTTCCTTCATTGGCCCTTCTCCCTACTATCCTCTCCTTTTTCTA22.8169014084507045No Hit
ACATAAACTTCATTCATAAATCATCAAAATAAAATAAAAACTAAAATTTT22.8169014084507045No Hit
CTTCATACTCTTAAATGTTTTTTATTCAAGTTTATGAATTTTACTTTATA22.8169014084507045No Hit
ATATTATATAAAAAAGATGGACAATCAAACCTATTTCTCGATTCAATAGA11.4084507042253522No Hit
CAACACAAGAGGGTCTATTCATCCGGACGACAAAAATAAAAATAAAAATA11.4084507042253522No Hit
AGGTATGTTATTTGCTTTCTTATCTCTGGGTTTTCATGGCCGTCCCGAGC11.4084507042253522No Hit
AAATTTATTAGTAATAAAATTTATATATGTCATTTTTTTTAATATAGTAT11.4084507042253522No Hit
AGATGGAGACTTCCATCTTTATCCTTTGGCTTTTGGCATTGTTGATTCTA11.4084507042253522No Hit
AGATGGAGACTTCCATCTTTATCCTTTGGCTTTTGGCATTGTTGATTCTG11.4084507042253522No Hit
ACTTGTAGAGAAAGCTGTTGAGATGTTAAGAGACGCCATTGATGAGGAAC11.4084507042253522No Hit
ATATATATATAGTGATATTTATAACTGCTAGTTTTGTACTTTTATCCCCA11.4084507042253522No Hit
AAATATAGACATATTTAAATGTTGGGCGATGCACTTGATGATGACCTATG11.4084507042253522No Hit
TGATAATAGTATGGTGGTGGTGGTGATTTCACCGGTGGGGGTGGGGAGTG11.4084507042253522No Hit
CTCCAAGGTCGTCTCGGAGATGCTTTTAAAGTTCTTGACAAAACATCAAA11.4084507042253522No Hit
ACTTTTCTGAACAGCGATCTCATTCTCGTTCACTGCTTTCTTCATCTACG11.4084507042253522No Hit
GACTTTGCTTATGTTATCTATTAGACTTTTGCTAGGTTAGTAATACAATG11.4084507042253522No Hit
GATTCAAAGAGATGCAAAAATTTGAAAGTTTGAAAGCTTTTGAGTTTTAG11.4084507042253522No Hit
AATTATACTAATAAGAAAGGAAGAAAAGGAAAAAAAAAGTAGAAGGCGAA11.4084507042253522No Hit
GACTTAAAATTTCTTTATATATAACGAATCAAAAACTCCGGATTCTCTAC11.4084507042253522No Hit
GCTTCGATCTTTTTCAACATAATCATCGCGCAGTAAATCAACAAAGCCCA11.4084507042253522No Hit
TCTCTAAGGCGTGGAACAGGAGGGCTAGGAAGTAGTATGGTGTTTGTGGC11.4084507042253522No Hit
TGTCATTACTGTTTTCCCAATTTTGGTTAAATATGTCCTGACTCAATATC11.4084507042253522No Hit
CTAGATTTGTTAATCACACTAGACTTAAGATTGATGACCTTAATTCTGGT11.4084507042253522No Hit
AAAATATCTTACATTATTTAGTCCCTACTTTACCATTTACTAACATATAA11.4084507042253522No Hit
TGGCTGGGTGGAGCGTAGCATAGCTTTACTTTTATGGTCTTAAATTCAGA11.4084507042253522No Hit
GCATCCATAATTACTCTAGCAAGACGAGGCATATACTCAACCGAGTAAGT11.4084507042253522No Hit
ATTACAGTTTGAGTGTTTAACCATCGTGTTATAAACTCTTAATTAGGAGC11.4084507042253522No Hit
GTGTCTTTCTCTAGTCTTTTTATTTGCCCATGAAAATTCGAGCTTATGCT11.4084507042253522No Hit
TCATTGGATAGTGTTGTTGCTCGTCATCTTCTATGGTACGTGTCATACGC11.4084507042253522No Hit
GTTTCAGAACACAAAAGTGAGGTTTATGAAGGAAAAAGGAGGATTTTATG11.4084507042253522No Hit
CCATCAGGTCCAGGTCTGCTAACAAAGCTAGAATCTACATAGAGCCTATG11.4084507042253522No Hit
AGCTGGTACTGAGTCCTTTTATGAAGCTTGGGAACACTATAAGGAATATC11.4084507042253522No Hit
GAAGCCTTGCTCCTTAACTGCGTACACCTGAGAAGCAAAAGAAGTTTGAA11.4084507042253522No Hit
CCTTACACTCATTGCGGTCTCTATGCTTAAGCAGAAACAATATTGAGACC11.4084507042253522No Hit
GTGGTGGTTTCCACAACAAATGCGGGATCACCTCAAAAGGACCTATAAAG11.4084507042253522No Hit
TAATCCAAATTTACAATTGATTAGAGTCTTTGGATACAAATTACGAGAAT11.4084507042253522No Hit
GTATGTTCTTTGAAATTGTGGTTTGATAACTCCGTTGGCATTAGTTGATA11.4084507042253522No Hit
GCCTCATCTCATTGTTAAGAAATAGTAGTATCCCTATCTAACACTAAGTC11.4084507042253522No Hit
GGTCTATGCAAGTCCCGGTGGGAGAAATTTGGTAAGAACACAGCCGGGAA11.4084507042253522No Hit
ATTATATCCTTACTGATATTAGGCACTATGAAATACGCATTTCTAAAGTA11.4084507042253522No Hit
ATCTTATGGGATGGGTAGATGTTATTATACAAAAACTATAATTTGCAATA11.4084507042253522No Hit
CATCAACGTCTTCTCCAGATTATTCTCATCTCTGATTTCAACTAACCGGT11.4084507042253522No Hit
CTAAGTTCTTCCACGGATCGGATATCGTGGCTGGAGTGTTCTTTGGCGGA11.4084507042253522No Hit
ATCGTCGAGAGGGAAACAGCCCGGATCACCAGCTAAGGCCCCTAAATGAT11.4084507042253522No Hit
CTAGCTTGTCGGTATTCTGACTCCGCGCTTGAATATGAGACCACCTTCTG11.4084507042253522No Hit
GCTGACGCTTTGACGAAAATCAACGACAGTTTGACAAGTCTGACCGCAAC11.4084507042253522No Hit
AGCCCATAGCGGTCGAAGTCAAATCGGGAGCCTATTAATGAGGCAAATGC11.4084507042253522No Hit
TGTCTACAGTTTTGCTTCCTGTTAAATTGATTTTTGTTCCCATCTTCTTC11.4084507042253522No Hit
ACAATAGAAAATACTCTAAAACCAATGCATATATCATACTAAAAACACGT11.4084507042253522No Hit
AAAGTGTTGGGCTCATCGAGGCTAAAACCAGAAGTGAGAAGAGCTGTCTC11.4084507042253522No Hit
CTTATTGAGGTTTGGTAATTGGAAAATTTGAGATCTGAGGCTATGAGGTT11.4084507042253522No Hit
GAAAGATGCAAGTGATAAGAAGATTCTAGTGGACATGTTGTTTTGGGCAT11.4084507042253522No Hit
GCCTGTAGATGAAGTTGGCTACACTGGTACTGGTGATGTCAAATATCACT11.4084507042253522No Hit
GAAATGGGAAGTGTACCGAGAATTTATAGTAAAAGGGTTCTAGTAGAGAG11.4084507042253522No Hit
AGCTAGTCCATGAACAGCCAACCAGCGCACTGTAAAAATTGGATAGGTCC11.4084507042253522No Hit
GCTTCGGGCAGTTTTTTCCTCGGACATTTATTTTAAAATTATTATAATTA11.4084507042253522No Hit
ATATCAAATGACAACAAGTTGAAAAAATTGACAAGACGTAACAAATATGT11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)