FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC_1wkTYM3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC_1wkTYM3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14605787
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTAT194263313.300433588412593TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGCGTAT2262181.5488244488297687TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGGAT1016870.6962103445709568TruSeq Adapter, Index 7 (97% over 36bp)
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC976960.668885558854172No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT966730.6618814857426033No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC744390.5096541528368174No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT713930.48879940533159905No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC499880.3422479048886582No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG480010.3286437081411635No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC439990.30124360981027587No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTTT437150.29929917504616493TruSeq Adapter, Index 7 (97% over 36bp)
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT425930.2916172884076702No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG424470.29061768462048637No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT401600.27495950748836745No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA369800.2531873154113503No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG368790.2524958086818601No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG350470.239952835133088No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT335990.23003895647663491No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC329850.22583514328943727No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC320790.21963212252787198No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG319070.21845450710735412No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT316200.21648953253939687No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC311020.212942993075279No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC307010.21019750596116457No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC303410.20773272949961546No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC281440.1926907464828838No Hit
GCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCA276130.18905520120209884No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT266110.18219490671745386No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC260940.17865521385461805No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC260180.17813487215717988No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA254780.1744377074648562No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC243860.16696121886482393No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG241530.16536596076609908No Hit
GCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCC238240.16311342894429448No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC232740.15934779823915No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG231370.15840981386350494No Hit
GCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTT226330.15495912681733617No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT224520.15371989198527952No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA221400.1515837523852703No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT219650.15038559716090616No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT219010.14994741467885297No Hit
GCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGA217810.14912582252500328No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT217180.14869448664423218No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT216380.14814675854166573No Hit
CGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCC214800.14706499553909694No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA213960.14648988103140215No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT213050.1458668403147328No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA210480.144107263785238No Hit
GGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT209790.14363484829677442No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC207640.14216282902112704No Hit
CTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGT206060.14108106601855827No Hit
GTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGA205830.1409235941890704No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC205040.14038271268778602No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC204200.13980759818009122No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG200350.1371716566864901No Hit
AGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAG198190.13569279080956062No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT196780.1347274200287872No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC195120.1335908842159618No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT188930.12935283802235376No Hit
CTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGC188860.12930491181337916No Hit
CAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAG185900.12727831783388324No Hit
AGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGA185170.12677851594029135No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT183100.12536126947490062No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT179410.12283487360181275No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA178650.12231453190437462No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA176440.12080143302103474No Hit
ACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTA175680.12028109132359659No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC174020.11914455551077117No Hit
GTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCAT170050.11642645480178507No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA167920.11496812872870185No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT167690.11481065689921399No Hit
CACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCC164560.11266767069792268No Hit
GTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTT163290.11179815233509842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCGCGGAT162030.11093548057355622TruSeq Adapter, Index 7 (97% over 36bp)
GTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATA161710.11071638933252963No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT161500.11057261070560594No Hit
CTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAA159820.10942238169021636No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA159250.10903212541713775No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC158900.10879249437226492No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT154940.10608124026456088No Hit
GTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACC154830.10600592765045799No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT153800.10530072771840367No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG151480.1037123162209609No Hit
CAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCA151200.10352061138506265No Hit
CTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGA150470.10302080949147074No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACC150340.10293180367480369No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA150180.10282225805429039No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC149700.10249362119275052No Hit
ACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACT148080.10138447178505341No Hit
GTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATG148020.10134339217736092No Hit
ACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTA147070.10069296505556326No Hit
GTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCC146540.10033009518761297No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)