FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC_1wkTYM1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC_1wkTYM1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190191
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTCGTAT3142813.0841522008763134TruSeq Adapter, Index 3 (97% over 36bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT684430.6716557128320755No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC679250.6665723930002883No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT498590.4892842538476462No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA485190.47613435312449No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC460540.45194442380913175No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG459400.4508257009117886No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG375210.36820703360712276No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCGCGTAT370250.36333960766780526TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG347720.34123011040715523No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC327880.3217604066498852No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT305310.29961165595424066No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC288460.2830761464628092No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT273960.2688467762773043No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT272800.2677084266624639No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG270190.2651471400290731No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG225800.22158564054393093No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA224820.22062393138656577No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC224490.22030009054786118No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA224240.22005475657914556No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA219910.21580557224099134No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC218490.21441207529868675No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG214910.21089889286667932No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC214180.21018251767802978No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG211500.20755253753339853No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC209640.20572725280615448No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG208980.20507957112874528No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA202290.1985144341259158No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC196390.19272455246422762No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC185530.1820672448632219No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG181100.17771992693758146No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC179750.1763951235065172No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA177620.17430487809306028No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC175190.17192023191714464No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC174270.17101740291227122No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC170150.16697429910783812No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT169030.16587520292799224No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC167250.16412842507073713No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT166590.16348074339332797No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT163000.15995774760257192No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA160220.15722963387045444No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT159390.15641512509431865No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC159280.1563071781480838No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA157320.15438375983335345No Hit
GCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCA154650.15176359304747084No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC153400.15053692320389284No Hit
GTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGA153080.15022289572393688No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC151760.1489275323691185No Hit
GCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCC150660.14784806290676986No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG150620.14780880947177535No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC149060.14627792550699No Hit
CGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCC148230.1454634167308542No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT147390.14463909459596977No Hit
GCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGA146370.14363813200361014No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT144660.14196004765759543No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA143740.14105721865272203No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT143480.1408020713252578No Hit
AGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAG140930.13829966484435866No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG139220.13662158049834394No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT137860.13528696370853108No Hit
CTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGC137500.13493368279358062No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT137110.13455096180238427No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA135500.1329710110438558No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT134840.1323233293664466No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT134180.13167564768903742No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACC131780.12932044158936765No Hit
CTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGT130570.12813302518078415No Hit
GTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATA130030.12760310380835843No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA129300.1268867286197089No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA129190.12677878167347403No Hit
CTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAA128710.12630774045354007No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC128680.1262783003772942No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG127900.12551285839490153No Hit
ACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTA126140.1237857072551437No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT126040.12368757366765745No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGC125310.12297119847900789No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC125080.12274549122778954No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG117410.1152186450675949No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC117400.11520883170884628No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT116150.11398216186526827No Hit
CACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCC116080.11391346835402791No Hit
AGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGA114630.11249053133547743No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA114490.11235314431299669No Hit
CAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAG114280.11214706377927558No Hit
CCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCA113210.11109703439317281No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG112980.11087132714195445No Hit
GGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT111280.10920305615468838No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT110860.10879089508724615No Hit
CTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGA110830.10876145501100029No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA110490.10842780081354708No Hit
CCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACA110440.10837873401980395No Hit
CAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCA109500.10745627829743327No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA109010.10697542371875071No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT108800.10676934318502959No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC108550.106524009216314No Hit
CCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCG107780.10576838059266995No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC107560.10555248670020023No Hit
CCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTT106450.10446320387910296No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG106310.10432581685662222No Hit
CCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACG106160.10417861647539287No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT105960.10398234930042037No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA105010.1030500802193011No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC103800.10186266381071757No Hit
GTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCAT102890.1009696481645928No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA102050.10014532602970838No Hit
ACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACT101980.10007663251846802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)