FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC_1wkDIP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC_1wkDIP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14327357
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTAGAAATCTCGTAT162953111.373563177074459TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTAGAAATCGCGTAT1508221.0526854324911425TruSeq Adapter, Index 2 (97% over 37bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT980840.6845924199417939No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC947970.6616502960036523No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT769850.5373286922354207No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG574140.4007298764175416No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC513580.35846108950869304No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC428070.298778064928514No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT374350.26128336161372956No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG338000.23591231795229226No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC323490.22578483945084915No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT322030.22476580991176529No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC319580.22305579458933006No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC310340.2166065939447171No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA308540.2153502561568055No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA304210.21232806581144031No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC304100.21225128961329015No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC294310.20541820797792643No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG280510.1957862849372707No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG278470.1943624354443042No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT274550.19162641092840782No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC264340.18450018380919803No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC263320.1837882590627148No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC258690.180556679086031No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC251890.175810514109476No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT250600.17491013869480604No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC249920.17443552219715053No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG232000.16192798155305269No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT231610.1616557750323385No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC229470.16016212899559912No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT225510.15739818586219356No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG224710.15683981351201062No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT224620.15677699662261504No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT221920.1548924899407476No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG221730.15475987650757916No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT220730.1540619110698505No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG218990.1528474512082026No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA207160.14459052007987236No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT202200.14112861150873812No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT201970.14096807945806056No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA199820.1394674537669439No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA199710.13939067756879372No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC197130.13758992673945375No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA195970.1367802868316885No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA188260.13139897330680042No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT186340.13005887966636137No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC186150.12992626623319292No Hit
CTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGT185150.12922830079546424No Hit
GCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCC182050.12706460793850533No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC180340.12587108703998928No Hit
CGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCC180090.12569659568055713No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA178310.12445421720140008No Hit
GCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCA178100.12430764445947708No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG175180.12226958538130933No Hit
CACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCC174820.12201831782372702No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA174560.12183684680991756No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT170070.1187029819945158No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACC169070.11800501655678712No Hit
CTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAA168210.11740476628034047No Hit
GGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT167320.11678357704076195No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC167140.11665794326197079No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC166510.11621822503620173No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG165730.11567381199477336No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC165100.1152340937690043No Hit
CTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGC163670.1142360031930523No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA160670.11214210687986625No Hit
AGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAG152750.10661422061305516No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT152540.10646764787113214No Hit
GTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGA150610.10512057457631578No Hit
CTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGA150200.10483440874684703No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA149660.10445750741047355No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT146980.10258696003736069No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG145870.10181221840148186No Hit
GCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGA144780.10105143607435763No Hit
CTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGA143690.10029065374723337No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC143570.10020689789470591No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)