FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC_1wkDIP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC_1wkDIP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24657227
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGGAGCATCTCGTAT7676293.1132008477676747TruSeq Adapter, Index 18 (97% over 38bp)
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC1296750.525910719806408No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC1050450.4260211417934385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGGAGCATCGCGTAT841670.3413481978326273TruSeq Adapter, Index 18 (97% over 38bp)
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC808400.3278551963689996No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT795400.3225829084511409No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT776780.315031369910331No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG746360.30269421618254155No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT738710.2995916775231862No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC715160.2900407251796806No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC701700.28458187938165147No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA673340.2730801805085381No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT640680.25983457101644075No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC630610.2557505756831455No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT611500.24800031244389323No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG598740.24282535907221037No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC597350.24226162982560856No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG593170.24056638647971243No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT555720.22537814167018863No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC555520.2252970295483754No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC545890.22139148088306929No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG523050.21212847657200057No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC504260.20450799272764938No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA498730.20226524255951409No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC498400.20213140755852232No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG479860.19461231386643763No Hit
TCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGC459090.18618882001613563No Hit
GCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCC431460.1749831803876405No Hit
GCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCA429850.17433022780704416No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT423980.1719495870318264No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG422720.17143858066440318No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT419160.16999478489612802No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA415360.168453654581677No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC412740.16739108578592393No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT412250.16719236108748156No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG412160.16715586063266563No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC407020.1650712791020661No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT405210.16433721439965654No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT402070.16306375408718912No Hit
AGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAG399500.16202146332188935No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG393690.15966515618321558No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC393600.15962865572839963No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC393390.15954348800049575No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT388880.15771440965360786No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC387470.1571425691948247No Hit
GCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGA383730.15562577251691767No Hit
CGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCC383680.15560549448646435No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA378850.1536466367446753No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT368060.14927063777285257No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA365920.1484027380694512No Hit
GTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGA364500.1478268420045774No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT356360.1445255786467797No Hit
CTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGT354850.14391318212708995No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC349470.14173126605031458No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT343120.13915595618274512No Hit
CTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAA335300.13598447221984858No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA333070.13508007206163125No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC326600.13245609492097388No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA325360.13195319976573197No Hit
CAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCA323520.13120696824505043No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT318000.1289682736830058No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC313740.1272405854883844No Hit
CACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCC309610.1255656201729416No Hit
ACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACT307290.12462471955990834No Hit
CAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAG297650.12071511528851156No Hit
GGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAAT297210.12053666862052248No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA289840.11754768693170567No Hit
CTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGC288660.11706912541300772No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA287220.11648511813595258No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC286460.1161768920730624No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT285920.11595788934416673No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA283120.11482231963878176No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG279570.11338257947659726No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT278620.1129972968979845No Hit
ACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTA278390.11290401795789931No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA272610.11055987763749751No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACC270170.10957030975137635No Hit
GTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTT264540.10728700352233445No Hit
GGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGAT260050.10546603638762785No Hit
ACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTA258530.10484958426184746No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG258410.10480091698875953No Hit
GCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGG254540.10323139743167388No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA254540.10323139743167388No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT254520.10322328621949256No Hit
CCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCG253520.10281772561042651No Hit
AGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGA251900.1021607174237395No Hit
CACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCT248220.10066825438237641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)