FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11110143
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGGATATCTCGTAT4954584.459510557154845TruSeq Adapter, Index 15 (97% over 37bp)
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT733580.6602795301554625No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC640890.5768512610503753No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT584600.5261858465728119No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG498570.448752099770453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATATGGATATCGCGTAT484540.4361239994840751TruSeq Adapter, Index 15 (97% over 37bp)
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT445560.40103894252306205No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT386970.34830334767068255No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT377220.33952758303831015No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC334600.3011662406145448No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA332780.2995280978831686No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC298510.2686824102984093No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT287810.2590515711633955No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC281930.25375911003125706No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG280790.25273302062808733No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC269270.24236411718553036No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT266250.23964588034555448No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC263290.23698164821100862No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC253910.22853891259545447No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT251210.22610870085110513No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG247160.22246338323458123No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT238770.2149117252586218No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC238650.21480371584776178No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA237260.21355260683863386No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT234910.21143742254262615No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG234840.2113744170529578No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT234770.21131141156328948No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA234530.2110953927415696No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG234140.21074436215627468No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC230520.20748607826199897No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT227070.20438080769977487No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG226480.20384976142971337No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA221320.1992053567627347No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC220250.19824227284923335No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC219380.1974592046204986No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC209840.18887245645713113No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA203120.1828239294489729No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT200140.18014169574595035No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA195520.17598333342784156No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT194590.17514626049367682No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC193860.1744892032442787No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC192890.1736161271731606No Hit
CCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTG192720.173463113841109No Hit
CCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTG192440.17321109188243572No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC188920.17004281583054331No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT187030.1683416676094988No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGT186560.16791863075029728No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA186560.16791863075029728No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT184740.1662804880189211No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC182610.16436332097615663No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT179770.16180709825247072No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC173490.15615460575079906No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA172870.15559655712802256No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT171310.154192434786843No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC170420.15339136498963155No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG170150.15314834381519662No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA170130.15313034224671995No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG169310.15239227793917684No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA165750.14918799875033112No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG165140.14863895091179297No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT165100.14860294777483962No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA165060.14856694463788628No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT163240.14692880190651011No Hit
GGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCG162980.14669478151631352No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT161480.1453446638805639No Hit
CCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGAT161370.14524565525394229No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA160880.14480461682626408No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT160600.1445525948675908No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA160380.14435457761434753No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC159160.1432564819372712No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA157730.1419693697911899No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT156450.14081726940868358No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC153840.13846806472247927No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT153750.1383870576643343No Hit
GCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTT151260.13614586238898996No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA150950.1358668380776017No Hit
CAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGC150890.13581283337217173No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA150560.13551580749230682No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG149400.13447171652066045No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC149220.13430970240437048No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT148400.13357163809682737No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA148190.13338262162782244No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA147140.13243753928279772No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT145820.13124943576333806No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT145600.13105141851009477No Hit
CTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCA145200.13069138714056155No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC144820.130349357339505No Hit
CGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATC143230.12891823264561042No Hit
GCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTA143130.12882822480322711No Hit
GGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT142870.12859420441303052No Hit
CCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTT142440.1282071706907823No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG142230.12801815422177734No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT141540.12739710010933253No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC139640.12568695110404968No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA136660.12300471740102714No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA136500.12286070485321386No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG136450.1228157009320222No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA135770.12220364760381572No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT135660.12210463897719408No Hit
CCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACA133930.12054750330396287No Hit
CTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCC132490.11925139037364325No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT131480.11834231116557185No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG131210.11809928999113692No Hit
CCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAA130800.11773025783736538No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG130750.11768525391617371No Hit
GCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAG130200.11719021078306553No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT130020.11702819666677558No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT129260.11634413706466244No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA129240.11632613549618577No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC129180.1162721307907558No Hit
GCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGG129090.11619112373261083No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG128860.11598410569512922No Hit
CTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCA128850.11597510491089089No Hit
CGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATC127390.11466099041209461No Hit
TCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGT127320.11459798492242629No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC126670.11401293394693479No Hit
GCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCC126480.11384191904640652No Hit
CTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAG125400.11286983434866679No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA124930.11244679748946525No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC123430.11109667985371564No Hit
ACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACT122880.11060163672060747No Hit
ATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGC121820.10964755359134443No Hit
CTCATCTTTTAGTAGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGG121590.10944053555386282No Hit
GTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTC119630.10767638184315No Hit
CTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGG118490.1066502924399803No Hit
CTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCC118220.10640727126554536No Hit
CTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGC117890.10611024538568045No Hit
CAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCA117670.10591222813243718No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG116930.1052461700988007No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC116890.10521016696184739No Hit
GGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAAT114930.10344601325113457No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG114500.10305897952888636No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC113830.1024559269849182No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT113340.10201488855723999No Hit
GCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACC112560.1013128273866502No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA112480.10124082111274355No Hit
CCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATAC112430.1011958171915519No Hit
CTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGT111960.10077278033235036No Hit
CCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTT111840.10066477092149038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)