FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Nov_G3_DIP_PC1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Nov_G3_DIP_PC1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7603203
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCTCGTAT2120522.7889824854077943TruSeq Adapter, Index 25 (97% over 38bp)
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC711400.9356583008503127No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT654430.8607293531423532No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT613840.8073439575399999No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG453650.5966564354522692No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT260580.3427239809327727No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT247140.32504722023073696No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT246590.32432384088653166No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT245250.3225614257570132No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA224570.2953623624148928No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC219850.2891544524064398No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG218750.28770769371802907No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGATATCGCGTAT203630.2678213379282389TruSeq Adapter, Index 25 (97% over 38bp)
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA199720.26267876840852467No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC197970.2603771068587804No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG191620.2520253635211371No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT186440.24521244533389414No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC172820.22729894230102762No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT168940.22219582983645184No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG167770.2206570046860514No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC167010.21965742595587676No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG164080.21580378690401925No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG151340.19904769082188123No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC151000.19860051086364522No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGT148190.19490470003234164No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA147590.1941155589295722No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT147300.19373414072990028No Hit
GATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCT147010.19335272253022837No Hit
CCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTG145860.1918402020832536No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC144780.1904197480982686No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT141930.18667132786011367No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA141530.18614523379160072No Hit
GGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCG140550.18485630332374395No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC139810.18388302929699496No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGT138890.18267301293941512No Hit
CCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTG137910.18138408247155838No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA137520.18087114075475821No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC136750.17985840967287078No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT131800.17334799557502278No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC131470.1729139679684996No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC130160.17119100989411962No Hit
GCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGATTT129790.17070437288074514No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT128340.16879728188238563No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA127180.167271609083698No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT127000.1670348667528672No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT126940.16695595264259025No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA126510.16639040151893877No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC126240.16603528802269255No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT125810.1654697368990411No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA125440.16498309988566662No Hit
GATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGA125340.16485157636853837No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA125100.16453591992743058No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC122830.1615503360886195No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC122410.16099793731668088No Hit
CCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGAT122220.16074804263413722No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT121000.15914345572517266No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG120060.1579071346641672No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT119850.15763093527819788No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG119580.15727582178195165No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA119470.15713114591311056No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC117560.15461904673596116No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC116010.1525804322204734No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC115920.152462061055058No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA115810.15231738518621693No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA115260.15159400584201158No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT112520.14799026147269775No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC112300.14770090973501562No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT112250.1476351479764515No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT111440.14656980748771273No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT109160.14357107129718882No Hit
GCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTA109070.14345270013177341No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT107190.14098005800976246No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT105230.13840219707404894No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT104580.13754729421271536No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC104500.13744207539901276No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG104110.1369291336822126No Hit
GGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTT103030.1355086796972276No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT102840.13525878501468394No Hit
TCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGT102630.13498258562871462No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC102460.13475899564959662No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG101890.13400931160196564No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA101580.1336015886988681No Hit
GCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCC101180.13307549463035515No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT100540.13223374412073438No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG100320.13194439238305225No Hit
CAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGC99460.13081329013574935No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG99420.13076068072889807No Hit
ACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACT98640.1297347972952978No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA98410.12943229320590283No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA97900.1287615232685488No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG97520.1282617339034615No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC97250.1279066204072152No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC96610.12706486989759447No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC95830.1260389864639942No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA95650.12580224413316335No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC94360.12410559076220903No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA93620.12313231673546003No Hit
CCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTT93610.12311916438374722No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG93170.12254046090838297No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT92810.12206697624672128No Hit
GCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGG92570.1217513198056135No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT91610.12048869404118238No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA91010.11969955293841293No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG90640.11921291592503842No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA90470.11898932594592042No Hit
GGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAAT89340.11750311020237128No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG89120.11721375846468916No Hit
GTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCC89030.11709538729927373No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG87730.11538558157660658No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG87730.11538558157660658No Hit
GTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTT87160.11463589752897561No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA86030.11314968178542649No Hit
GCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAG85760.11279456828918023No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC85580.1125578259583494No Hit
CCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACA84360.11095323904938485No Hit
CGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATC83890.1103350785188821No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC83270.10951963271268701No Hit
GCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTC82900.1090329956993125No Hit
GTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTC82730.1088094057201945No Hit
GGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGAT82260.10819124518969177No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA82120.10800711226571222No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC81870.10767830347289162No Hit
CCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAA81850.10765199876946598No Hit
GCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACC81750.10752047525233774No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC81630.10736264703178383No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA81310.10694177177697348No Hit
CCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCG81080.1066392676875785No Hit
GTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTC80290.10560023190226542No Hit
CTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCC79960.1051662042957422No Hit
GCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATC79530.10460065317209077No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC79500.1045611961169523No Hit
ACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTA79290.10428499673098299No Hit
CTCATCTTTTAGTAGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGG78990.10389042617959825No Hit
CTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCA78850.10370629325561873No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG77390.10178604990554638No Hit
GTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCA77380.10177289755383355No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG77340.10172028814698228No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT76830.10104951820962824No Hit
CTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCC76710.10089168998907434No Hit
CAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCA76610.1007601664719461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)