FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13721949
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCATCTCGTAT2861102.0850536611089288TruSeq Adapter, Index 1 (97% over 37bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT515540.3757046466212635No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA419650.3058239030038663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGGCCATCGCGTAT347240.25305443126191474TruSeq Adapter, Index 1 (97% over 37bp)
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC316030.23030984884144373No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG305200.22241738400281186No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC299770.21846022019175265No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT279640.20379029247230113No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT271450.19782175258048254No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC270490.1971221435089141No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC268270.19550429753091197No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT264270.19258925973271No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG261220.19036654341158096No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC257110.18737134207392842No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC250040.18221901276560643No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA244690.17832014971051124No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG241580.1760537078224092No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA238550.1738455666902712No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA234370.1707993521911501No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT233920.1704714104388524No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC228350.1664122203048561No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC225870.16460489686997087No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT218400.15916106378182865No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT216320.15764524412676362No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC215720.15720798845703332No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT215350.15693834746069965No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC214250.1561367120661941No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA213170.15534965186067956No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA212190.15463546760012006No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC208490.15193905763678323No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT206080.15018274736336654No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT205890.15004428306795192No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT205040.14942483753583402No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC203860.14856490138536443No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA202260.14739888626608363No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC196670.14332512094309635No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG195750.1426546622495099No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC195270.14230485771372564No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG195080.14216639341831105No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG193070.14070158692471454No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA192140.14002384063663262No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA190110.13854445895404507No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT188730.1375387709136654No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC184800.13467474627693196No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG183170.13348686837416462No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT175300.12775153150620222No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG174570.12721953710803036No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC172100.12541950126764062No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG168270.12262835257586223No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG167890.12235142398503303No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC167420.12200890704374429No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT166400.12126557240520279No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA162660.11854001206388393No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA162450.11838697257947832No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA159100.11594562842348415No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC158780.115712425399628No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG158510.11551566034824937No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG158440.11546464718678083No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA157480.11476503811521235No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG155520.11333666959409339No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA154450.11255689698307435No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT152390.11105565251700031No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC151850.11066212241424304No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG146830.10700374997749955No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG146410.10669767100868835No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA146350.1066539454417153No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT146140.10650090595730971No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG144500.10530574046004688No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC142840.10409599977379307No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT140840.10263848087469207No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC140400.10231782671688985No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG140320.10225952596092582No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC139250.10147975334990679No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT139100.10137043943247422No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA138310.1007947194673293No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA137370.10010968558475185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)