FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53614312
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAATTATCTCGTAT10121561.8878466630328112TruSeq Adapter, Index 5 (97% over 37bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT2449650.456902254010086No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA2165890.40397608757900316No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC1669650.3114187122274366No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG1528690.28512722498425425No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC1481040.2762396727202244No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC1389250.2591192441301867No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC1352330.25223302315247464No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC1341600.2502316918661569No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG1322860.24673635651614817No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT1302480.2429351326936733No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT1256650.23438704202713634No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA1255390.23415203015194896No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC1249730.23309634188721848No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT1218520.2272751350422999No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA1195150.2229162243096582No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT1166900.21764710885406865No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC1104580.2060233469003575No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT1095320.2042961961350917No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT1094930.2042234543642004No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1089920.2032890023842887No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1084960.2023638762724401No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA1046680.19522399168341467No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC1045390.1949833842873895No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA1040600.19408996612695503No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA1015080.1893300430676048No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC1002680.1870172277879832No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA977190.1822629002494707No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG975190.1818898655269511No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC962790.17957705024732948No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG957930.1786705758716068No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA955690.17825277698238484No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC933370.17408970947906596No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG926670.17284004315862525No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT891850.16634550863955877No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA887970.16562182127787073No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC886970.16543530391661093No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT886780.16539986561797157No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC873220.16287069019928857No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT849010.15835510488318866No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA838210.15634071738158273No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG832900.15535031019329315No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA832810.1553335236307798No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC820680.15307106803869833No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT803710.1499058684181194No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG801240.1494451705358077No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG790670.14747368202729152No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA790070.14736177161053565No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA786140.1466287583807846No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG784540.1463303306027689No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA773530.14427677445529843No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG769680.14355868261444818No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC769450.14351578362135842No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC769250.14347848014910647No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG763770.14245636500940273No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC759670.1416916438282375No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC756130.1410313723693778No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG754800.14078330427890223No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT749630.13981900952118903No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT729340.13603457226122756No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA727310.13565594201787015No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC716730.1336825883357414No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC715020.13336364364798714No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA713440.13306894621719664No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT711840.13277051843918092No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG711030.13261943937656048No Hit
CTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGG710980.1326101135084975No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA708720.13218858427205035No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG695350.12969484715200671No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC690280.1287492041304195No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA688660.12844704600517862No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA686560.12805535954653302No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA682360.12727198662924183No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC679750.12678517631635372No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA667870.12456935006458723No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC663110.12368152742499056No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT658890.12289442416047416No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA656940.12253071530601753No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC650780.12138176836065714No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT638710.11913050381025125No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG638480.11908760481716152No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC631150.11772043255912712No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA629110.11733993714215711No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT627060.11695757655157452No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG621060.11583847238401568No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT618110.11528824616829925No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC604410.11273295831903989No Hit
CAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTC604060.11266767724259896No Hit
GCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAA602740.112421474325736No Hit
GGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTG597430.11143106713744644No Hit
ATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTAC596130.11118859456780868No Hit
GGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCG594740.11092933543565754No Hit
CGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTT594050.11080063845638828No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT592040.11042573856025607No Hit
GCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTAC591630.11034926644213955No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT580280.10823229439184076No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA578820.10795997904440144No Hit
GATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAAT575330.1073090334536047No Hit
CTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCAT566430.10564902893839241No Hit
GTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCAC562340.10488617293083981No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA560520.10454671133334696No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT559300.10431916015260999No Hit
GGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTAC549980.10258081834566861No Hit
CACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTA546460.10192427723403408No Hit
GTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAAC542470.10118007296260743No Hit
GGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGA540870.10088164518459175No Hit
GTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGG540430.10079957754563745No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC538960.10052539702458552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)