FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20580878
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCTAGATCTCGTAT1144090.5558995102152591TruSeq Adapter, Index 2 (97% over 36bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT852440.4141902983925176No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA674850.32790146270727616No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG573640.278724746339782No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC555930.27011967127933023No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC515590.2505189525927902No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC501910.24387200584931315No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT493560.23981484171860887No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT483600.2349753980369545No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC483270.2348150550234057No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG464000.22545199480799605No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA460400.2237027982965547No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC451720.21948529115230164No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT445550.21648736268685911No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT443950.2157099420151074No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT436690.21218239571703404No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT419690.20392230107967213No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA398250.1935048640781992No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT388400.18871886806772772No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC371090.1803081481752139No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA364060.17689235609870482No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT360430.17512858294966815No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC356060.17300525273994627No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA352650.17134837493327545No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG346020.16812693802470427No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC345230.1677430865680269No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA341610.16598417229818863No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC339390.16490550111613314No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC339090.1647597347401797No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT337920.16419124587396125No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT334000.16228656522816956No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC333030.16181525394592008No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA329720.16020696493123374No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG327370.15906512831959843No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA325270.1580447636879243No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT319630.15530435581999952No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA295380.14352157376376268No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG293840.14277330636720162No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC293750.14272957645441559No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA289730.14077630701663943No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT288080.13997459194889547No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC285990.1389590861964198No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG285940.13893479180042756No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC284840.13840031508859826No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA284160.13806991130310378No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC276470.13433343319949714No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG272360.13233643384893493No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA270820.1315881664523739No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA267570.13000903071287823No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG266210.1293482231418893No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA261360.1269916667306419No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT256470.12461567480260075No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA254970.12388684292283351No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA254770.12378966533886455No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA249250.12110756402132115No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG247080.1200531872352579No Hit
CCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACA246850.11994143301369357No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT245920.11948955724823791No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG245030.11905711699957602No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA244310.11870727769728776No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC243890.11850320477095291No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC239530.1163847334404295No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT239420.11633128576924658No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG237830.11555872397669331No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG237440.11536922768795384No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG235470.11441202848585955No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC234090.11374150315647369No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA233000.11321188532384284No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC232700.1130661189478894No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT232070.11276000955838716No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT231810.11263367869922751No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC229140.11133635795324184No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG227400.11049091297271187No Hit
GCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTAC227380.11048119521431495No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA226220.10991756522729496No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC224780.10921788662271842No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA223260.1084793369845543No Hit
GTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCA221090.10742496019849104No Hit
GTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGG216590.10523846455918937No Hit
GTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCAC216570.10522874680079246No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC216010.10495664956567936No Hit
GGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTG215380.10465054017617713No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA215140.10453392707541437No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC215060.10449505604182678No Hit
GCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAA212090.10305196891988767No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT210950.10249805669126458No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT209430.10175950705310045No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT207560.10085089664299063No Hit
GTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAAC206010.10009777036723118No Hit
GTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGC205990.10008805260883429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)