FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35055693
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCTCGTAT7351072.0969689573673524TruSeq Adapter, Index 1 (97% over 36bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT1694560.48339081472444434No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA1564970.4464239232127004No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG1155900.3297324631408656No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT1056910.3014945389897156No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC1025120.2924261117873208No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC1020880.2912166078131732No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG1013230.28903436597302473No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT989650.2823079264186847No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT982690.28032251423470644No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC981150.27988321326296417No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC911710.2600747330825838No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT890680.2540757074749599No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC881600.2514855433039079No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT856800.24441108609662915No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT821980.23447831996931284No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC807080.23022794043752037No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC792120.22596044528345227No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT778770.2221522193271147No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT775730.22128502779848055No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC772720.22042639408098422No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA772610.22039501544014548No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA749920.21392245761622797No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA748830.2136115238115532No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC723710.20644578328547092No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTTCCATCGCGTAT714910.20393549201837202TruSeq Adapter, Index 1 (97% over 36bp)
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT706090.20141949554384792No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG700610.19985626870933632No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC693820.1979193507884725No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA685310.19549178502903936No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC673660.19216850170384595No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC672650.1918803887288721No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA666190.19003760673052447No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC660920.18853428457397775No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC656670.1873219279961175No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT651130.18574158553933023No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC640060.18258375322946832No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA638220.18205887414634764No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA637910.18197044343125665No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC635300.18122591386226483No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA635160.18118597741028825No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG625260.178361899734802No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA604110.1723286428826268No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT599470.17100503475997467No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG596890.17026906300212066No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA582120.16605576731859215No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT574710.1639419879675464No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG572910.16342851929927615No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA561280.16011094118150795No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA549740.15681903649715326No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG545320.15555818565617857No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT539810.15398640101052916No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT536470.15303363137051662No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG536460.153030778766804No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA531280.1515531300436708No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA530770.15140764725432757No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT516280.14727422447475222No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC514590.14679213444732073No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA514160.1466694724876784No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG513900.14659530479115046No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG511020.1457737549219181No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG507680.14482098528190557No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA505190.14411068695746507No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG502520.14334904176619756No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG501590.14308374962092463No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA498290.14214239039576254No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA490720.13998296938531496No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA486400.1387506445814664No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG484800.13829422798744842No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT480440.13705049276874942No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA477920.1363316366331711No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA473470.1350622279810586No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT471860.134602958783328No Hit
CTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGG470900.1343291088269172No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC470760.13428917237494065No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC469700.13398679638140373No Hit
GCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAG463620.1322524133241354No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC459310.13102294112399945No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC454930.12977350069787524No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG452760.12915448569223834No Hit
CTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCA448480.12793357130324023No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA446490.1273659031644304No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC445290.1270235907189169No Hit
GCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAG444620.12683246627017186No Hit
GTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTC444170.12670409910310432No Hit
GCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAA443260.12644451216525657No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC436000.12437352186989999No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT434410.12391995787959462No Hit
GCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACC433350.12361758188605772No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC430550.12281885284652624No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT428460.12222265867059025No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT426330.12161505407980382No Hit
GGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGA423900.12092187137763899No Hit
CAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTC419800.11975230385546792No Hit
GCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTAC417970.11923027737605987No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG417140.11899351126791304No Hit
GTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGG413730.11802077340191221No Hit
ATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTAC411890.11749589431879154No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT411570.11740461099998795No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG408040.11639764188943576No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG406200.11587276280631507No Hit
GTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCAC404420.11536499934547008No Hit
GCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTC404150.11528797904522955No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT403980.11523948478211514No Hit
GTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTC402560.11483441505492419No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA399470.11395296050772695No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC398690.11373045741814318No Hit
CTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGC396670.11315423146819549No Hit
CTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGC391880.11178783428985414No Hit
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG389460.11109750419140195No Hit
GGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTG389190.11102048389116141No Hit
GTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTC389040.11097769483547222No Hit
GATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAAT388120.1107152552939119No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC387980.11067531884193531No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT387200.11045281575235155No Hit
GTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT385500.10996787312120744No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT383530.1094059101898228No Hit
CCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGA383100.10928324823018049No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT382290.10905218732945887No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC381330.10877833737304807No Hit
CTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTG380500.10854157126490124No Hit
GTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCC379610.10828768953447875No Hit
CTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACAT372310.10620528882427172No Hit
CTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGT370120.1055805686112096No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC370040.1055577477815087No Hit
CCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCAC368690.10517264628030602No Hit
GTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATC364840.10407439385095026No Hit
CGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATC363590.1037178183868737No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGT361960.1032528439817179No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG360850.1029362049696179No Hit
CTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTA360760.1029105315362044No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT360580.10285918466937738No Hit
AGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCC360490.10283351123596388No Hit
CTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCC360120.1027279648985972No Hit
CACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAA353760.10091370893737574No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTC353660.1008851829002496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)