FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19139829
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCTCGTAT1986761.0380239029303762TruSeq Adapter, Index 3 (97% over 37bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT812910.42472166287379054No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA721840.37714025553728825No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC680310.3554420470527715No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC630500.3294177811097476No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT619720.32378554688236766No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT526940.27531071463595624No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG517520.2703890405708431No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA512310.26766696818451197No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC489020.25549862540569196No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT478810.250164199481615No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG477770.2496208299457639No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC450370.2353051325589168No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC442100.23098429980748522No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT441690.23077008681739006No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT438060.22887351814898663No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC436310.22795919441077556No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG426730.2229539250324546No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC411430.21496012320695237No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA406990.2126403532654341No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA403690.21091619993052185No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA401280.20965704552532838No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT390070.2038001488937022No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT386600.20198717553850665No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT359680.18792226409128315No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC349880.18280205115730136No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA345350.18043525885210362No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA343160.17929104800257098No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA335670.17537774240302775No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA328150.17144876268225803No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA322700.1686012973261151No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA320610.16750933354733732No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC319290.16681967221337243No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC314650.1643954081303443No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC306790.1602887883690079No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG306580.16017906952042257No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC306570.16017384481334707No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC305170.15944238582277825No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG304790.15924384695390956No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA304630.15916025164070172No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT301210.15737340182088358No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA300940.1572323347298453No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC300100.156793459335504No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT299690.15657924634540885No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT294590.15391464573690808No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT292420.15278088430152642No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG288590.15077982149161312No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA283950.148355557408585No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA273600.14294798558545116No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA272880.14257180667601577No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA272840.1425509078477138No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT269680.14089990041185843No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC269150.1406229909368574No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG266480.13922799414770112No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG256050.13377862466796334No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC255370.13342334458682992No Hit
GTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCAC250390.13082144046323507No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA250250.1307482945641782No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA248420.12979217316936323No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT247810.12947346603775822No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT244170.12757167266227926No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA243840.12739925732878804No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG243180.1270544266618056No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC242840.12687678662123889No Hit
GTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTC241090.12596246288302784No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG239560.12516308270047763No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT237110.12388302946698218No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT234840.12269702096084557No Hit
GTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATC233150.12181404546508748No Hit
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG231720.12106691235329219No Hit
CTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGG230820.12059668871649794No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT230340.12034590277687433No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC230160.12025185804951548No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG230030.12018393685753409No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG229290.11979730853394772No Hit
CAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTC228320.11929051194762502No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA227960.11910242249290734No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT227480.11885163655328372No Hit
GGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTG226430.11830304231035711No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGACTCATCGCGTAT224730.11741484210752354TruSeq Adapter, Index 3 (97% over 37bp)
GCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTAC224240.11715883146082445No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC223320.1166781584098792No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT223260.11664681016742627No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG219810.11484428622638165No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA218240.11402400721552947No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA217920.11385681658911374No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC217860.11382546834666077No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA215580.1126342351334487No Hit
ATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTAC214400.11201771969854067No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT214260.11194457379948379No Hit
GCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAG212290.11091530650561195No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG211480.11049210523249713No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC211470.11048688052542162No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG210650.1100584545452313No Hit
GTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGG210600.11003233100985385No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT210570.11001665688862737No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC208580.10897694018060454No Hit
CTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCA205520.1073781798155041No Hit
GACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTG204020.10659447375418035No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA203380.1062600925013489No Hit
GTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCA203350.10624441838012241No Hit
GTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTC202760.1059361606626684No Hit
CCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTC202190.10563835235936539No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT200910.10496958985370246No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC200660.10483897217681516No Hit
GTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATT199570.10426947910558658No Hit
CTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATT199510.10423813086313362No Hit
CCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTC197300.10308347059944999No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT195050.10190791150746437No Hit
CCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTC194910.10183476560840748No Hit
CCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACA194440.10158920437585937No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG193980.10134886785038676No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC193800.10125482312302791No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG192130.10038229704142079No Hit
GGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATA191430.10001656754613639No Hit
GTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTA191400.1000008934249099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)