FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17478403
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGTCAATCTCGTAT2289121.3096848722391858TruSeq Adapter, Index 8 (97% over 38bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT756910.4330544386692537No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA596900.34150717316679335No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC529590.3029967898096868No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT525060.3004050198407715No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC481640.2755629332954504No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT468280.2679192143584285No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT454680.2601381831051727No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG452810.25906829130785003No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC452710.25901107784275257No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA448240.2564536359528957No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG419280.23988461646066864No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT407090.23291029506528715No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC401920.22995235891974797No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT391340.22389917431243578No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC368740.21096893120040772No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC364480.20853163758725557No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA363900.20819979948969022No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG362750.20754184464106934No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT361550.2068552830598997No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT349840.20015558629698604No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA347420.1987710204416273No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC335560.19198550348106747No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC323580.18513133036239066No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT321050.18368382969542468No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC317990.18193309766344215No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA315050.18025102178957655No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT311950.178477404371555No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT300710.17204661089459947No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA298540.1708050787019844No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT296680.16974090825117147No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA295440.16903146128396285No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA290870.16641680592900848No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA289720.16575885108038763No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC286760.16406533251350253No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG278570.15937954972201981No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC272080.15566639583719405No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT271540.1553574431256677No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA269520.15420173113069882No Hit
GTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCA269460.15416740305164037No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG268760.15376690879595808No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA266520.15248532717777477No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC266180.15229080139644335No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG264390.15126668037119867No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG261230.14945873487411865No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC256210.1465866189262257No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC255320.14607741908685823No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT250550.14334833680170894No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA247910.14183790132313576No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC245940.14071079606071563No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC245720.1405849264375012No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT244570.1399269715888803No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT242910.1389772280682623No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA241120.1379531070430176No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA239130.13681455908757795No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG238090.13621953905056428No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA235540.13476059569057883No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA233280.133467571379376No Hit
TACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAAC230070.13163101914974726No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG229570.13134495182425993No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG227630.13023501060136902No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT226110.12936536593188747No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG225840.1292108895761243No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA219870.1257952457098054No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT219400.1255263424238473No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG218310.1249027156542849No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG217070.12419326868707628No Hit
GTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCAC215260.12315770496881208No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC215060.12304327803861716No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA214380.12265422647595435No Hit
CCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACA214210.12255696358528866No Hit
CTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCG213820.12233383107140854No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA212020.12130398869965409No Hit
GTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTC209450.11983360264664912No Hit
GCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAG202500.11585726682237503No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT202020.11558264218990716No Hit
GCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTAC201440.11525080409234185No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT201140.11507916369704943No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA200520.11472444021344513No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC198610.11363166303008346No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT196380.11235580275840991No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA196250.11228142525378319No Hit
GCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTG195830.11204112870037382No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC195640.11193242311668863No Hit
GCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAA193500.1107080549636028No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA193470.11069089092407357No Hit
GGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTG190000.10870558368519137No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG189490.10841379501319427No Hit
GTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTT189420.10837374558762605No Hit
ATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTAT188310.10773867612504415No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC188120.10762997054135895No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA186710.10682326068348463No Hit
GTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATT186470.1066859483672507No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT186250.10656007874403628No Hit
GCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACC186130.10649142258591933No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG185370.10605660025117855No Hit
GTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGG184850.10575909023267172No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC182670.10451183669354688No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC182250.10427154014013751No Hit
CTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGG181910.10407701435880612No Hit
CTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTG181580.10388820992398447No Hit
CAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTC181250.10369940548916282No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT180300.10315587757073687No Hit
GCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTC178390.10206310038737522No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA178160.10193150941765104No Hit
CCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGA177700.10166832747820267No Hit
ATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTAC177250.10141086688526407No Hit
GCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATC176200.10081012550174062No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC175850.1006098783738995No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT175470.1003924672065291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)