FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18814212
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTAGTGATCTCGTAT8530744.534199997321174TruSeq Adapter, Index 16 (97% over 37bp)
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT761260.40461965667230704No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA723720.38466665518598386No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTAGTGATCGCGTAT693720.36872126241588005TruSeq Adapter, Index 16 (97% over 37bp)
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG509560.27083781133113627No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT504000.2678825985377437No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT467120.24828039569236277No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC454870.24176936031123705No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT453690.24114217486227965No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG443630.23579515315337152No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC437200.23237752396964595No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC436710.2321170825544009No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT419260.22284217909312384No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT412820.21941923477847491No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC405260.21540099580040878No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC404220.2148482221843785No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC402250.213801141392475No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA401940.21363637233385058No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT400440.21283910269534542No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC396690.21084592859908244No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT372950.198227807787007No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA371230.1973136052681877No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC366000.19453379179526625No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC362690.19277448345963147No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG358050.19030826271118875No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC351830.1870022512768539No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT350220.18614651519819167No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT343190.182409978159064No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA330500.1756650770173101No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG321840.17106217363767348No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT318060.1690530541486404No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG304940.1620796023771817No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC303310.1612132360366727No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC302080.16055947493309847No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG296900.15780623711479386No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA292260.15534001636635114No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA292090.15524965914065386No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT289260.15374547708934078No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT282940.15038631434577224No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG281150.14943490591048939No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG277640.1475692949563872No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT274560.1459322346319899No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA274080.14567710834766823No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC273790.14552296955089056No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA272940.1450711834224043No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA270530.14379023686987263No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA266060.14141437334712717No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA263650.14013342679459548No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC262820.13969227092795594No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC259810.1380924165200222No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG256220.13618428451853312No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT254610.13532854843987088No Hit
CCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTG250090.13292610926250858No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG248730.13220325145693054No Hit
GTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGA241050.12812123090778396No Hit
GTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTA239030.12704757446126363No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA238320.12667020016570452No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG235530.12518727863808488No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG235140.12497998853207351No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG233670.12419866428633843No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC233620.1241720886317216No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA232900.12378939920523911No Hit
CCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATT230950.12275294867518236No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT229140.12179090997805277No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG228380.12138696002787679No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC227230.12077571997168948No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT226780.12053653908013792No Hit
CTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCG225860.12004754703518808No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT225820.12002628651149462No Hit
CTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCA224140.1191333445163688No Hit
CTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGC218720.11625254355590336No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA218700.11624191329405664No Hit
CTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAA214030.11375974715284382No Hit
GGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAA213700.11358434783237269No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG213370.11340894851190153No Hit
CGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCAT213100.1132654399769706No Hit
CTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCA212340.11286149002679463No Hit
CCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTT212250.11281365384848434No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC212060.11271266636094034No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA209240.11121379944055058No Hit
CCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCC207790.11044310545666224No Hit
CACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGC206530.10977339896031788No Hit
GTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAA204930.10892297801257901No Hit
CCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTC203770.10830642282546833No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGT200730.10669062302476448No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA199970.1062866730745885No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA199740.10616442506335105No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG199360.10596245008826306No Hit
GCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCC197870.10517049558068124No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT195200.103751355624142No Hit
CTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGG192020.102061143990511No Hit
CCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCT191340.10169971508772198No Hit
CTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGT190930.1014817947198639No Hit
GTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGC190770.10139675262509No Hit
GCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAA190580.10129576513754601No Hit
CCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTG189290.10061011324843155No Hit
GTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTC188330.10009986067978825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)