FastQCFastQC Report
Mon 5 Feb 2024
HNCJWDRX3_n01_Dec_day3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCJWDRX3_n01_Dec_day3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9419966
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCTCGTAT8051888.547674163579783TruSeq Adapter, Index 21 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTCTGCATCGCGTAT811400.8613619199899447TruSeq Adapter, Index 21 (97% over 38bp)
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC311250.33041520532027396No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT305600.32441730681405856No Hit
CTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAAT283550.30100957901546566No Hit
CCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGT276080.2930796140877791No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT270940.2876231188095583No Hit
CCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCA261210.2772939944793856No Hit
CTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTG236400.25095631979988037No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG218000.2314233405937983No Hit
CCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCG212310.22538297908930882No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGC211270.22427894113418245No Hit
CTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATC197300.209448738986956No Hit
GTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAAC193560.20547844864832848No Hit
CCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATT190800.20254850176741618No Hit
CCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACC181700.19288816966006034No Hit
CTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCC179280.19031915826447782No Hit
CATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAG175710.18652933566851515No Hit
CCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGC173190.1838541667772474No Hit
CCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCA172450.1830686013091767No Hit
CCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAAC168630.17901338497400096No Hit
CCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCT165620.1758180443538756No Hit
CTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATC164970.17512802063192162No Hit
CGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCC162900.17293056047123737No Hit
CGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTA160760.17065879006357346No Hit
CTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATT160180.17004307658859916No Hit
GTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCT159990.16984137734679722No Hit
CCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCAC159820.16966090960413233No Hit
CTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTC158170.16790931092532604No Hit
GGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATA154150.16364177959877987No Hit
GCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGT153070.1624952786453794No Hit
CTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCA152050.1614124721893901No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACT147940.1570493991167272No Hit
CTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCA146450.15546765243101726No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCG145420.1543742302254594No Hit
CCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACAT143230.15204938106995292No Hit
GCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCAC142130.1508816486174154No Hit
CTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTC140520.14917251293688322No Hit
GTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCT140310.14894958219594423No Hit
GTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAA139350.14793047023736605No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTG138040.14653980704388955No Hit
CCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGT132600.1407648392786131No Hit
GTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTAT132390.14054190853767412No Hit
CTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTA129150.13710240567747273No Hit
GTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCC127750.13561620073787953No Hit
CCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCAT127400.13524464950298123No Hit
GTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTAC126150.13391768080691585No Hit
CCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTT126130.13389644930777883No Hit
GCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCC125140.13284549010049504No Hit
CCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATA124030.13166714189838902No Hit
CCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTG120210.1276119255632133No Hit
GCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAA118940.1262637253680109No Hit
CTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGA118350.12563739614346806No Hit
CCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTA117940.1252021504111586No Hit
CGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCC114490.1215397168100182No Hit
GTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAG113310.12028705836093251No Hit
CAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATT113270.12024459536265843No Hit
GTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCA113230.12020213236438432No Hit
CACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTG112490.11941656689631365No Hit
CCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTG110650.11746326897570543No Hit
GTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCT109710.1164653885162643No Hit
GGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTA108300.11496856782710256No Hit
CTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTT106020.11254817692547936No Hit
CCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGT105730.1122403201879922No Hit
GGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTT105510.11200677369748469No Hit
GGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTAT104670.11111505073372877No Hit
CTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCG101830.10810017785626827No Hit
CTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCA101340.10758000612741064No Hit
CTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGC101020.10724030214121791No Hit
CCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGA100990.10720845489251235No Hit
GGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAA100380.10656089416883245No Hit
CTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTT100270.1064441209235787No Hit
GTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCG98530.10459698049865573No Hit
CTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCG98440.10450143875253902No Hit
CTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGAC98100.10414050326720925No Hit
GTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGG97560.10356725279050902No Hit
CGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATC96480.10242075183710855No Hit
CCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTG96470.10241013608754002No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGC95720.1016139548699008No Hit
CCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCT95120.10097700989578944No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)