FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_z5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_z5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25198403
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT69000.011.50311
CGCGTAT17250.011.4030731
CCGGGAT125350.010.7368391
GGAAAAT114000.010.7160461
CGGCTAT34000.09.7438031
ATACCTA40100.09.4611328
GGGACAT47550.09.4347191
GGAAATT83950.09.2901751
TTAGCCG35350.09.2689178
CGTGACT21700.09.2236841
TCCCTAC44200.09.1298859
GGGAGAT112750.09.1208621
CTACACG39800.09.09926312
TTCCCTA47050.09.0898538
ACCTAGG36950.09.05442210
GGAAGAT210650.08.9447331
GCCGAAT34500.08.9023991
GAAAAAT147500.08.84373951
TACCTAG44350.08.632399
GGCGAAT32600.08.5743981
Produced by FastQC (version 0.11.2)