FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_z4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_z4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26483090
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG53250.012.8246567
ATACCTA54600.012.31915
CCTAGGT61200.011.4968518
CGGGACT30100.011.2354011
TACCTAG62050.010.84001356
GTGGATA69600.010.7591871
TAGGTAC67450.010.43159710
GGAAAAT120500.010.3955761
CGGCTAT33500.010.3011131
GGGAAAT80100.010.2966121
CTAGGTA70750.09.9938229
GATACCT73800.09.6750014
CCGGGAT124700.09.3812821
GGATACC79150.09.282483
GGGAAAC83450.08.8080991
CGACAAT64450.08.7811191
GGAAAAC74500.08.5692821
CTCGAAT45200.08.4744881
CGCGTAT20000.08.45463851
GGCGAAT34050.08.4118191
Produced by FastQC (version 0.11.2)