FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_z3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_z3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28755036
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT141150.011.6868981
ATACCTA53100.011.3021798
ACCTAGG49850.011.20881210
CCTAGGT53000.010.34735811
TACCTAG58400.010.0994289
GGGAAAT85200.09.6807841
GAAAAAT175600.09.1582741
GGGAAAC84700.09.1267641
TTAGCCG38650.09.1024478
CCGGGAT132300.08.9471861
CTCGGAT57000.08.9000961
TAGGTAC63350.08.87460913
CGGGACT36900.08.6977871
CGGAAAT42500.08.5261431
AAAATCC138500.08.5177883
CTAGGTA64700.08.47616912
GCCGAAT42400.08.4648591
CGCGTAT21200.08.4648591
GGAAGAT263450.08.4098651
CGGGGAT114200.08.3707791
Produced by FastQC (version 0.11.2)