FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_z2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_z2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27660313
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT148950.010.8199631
CCTAGGT52900.010.2363918
ATACCTA47850.09.8037995
CGGGCAT70550.09.7832341
ACCTAGG46900.09.7074417
GGGAAAT85800.09.4521381
CCGGGAT120400.09.4301561
CGACAAT74300.09.3823591
TTAGCGT57500.09.3574958
CGGGGAT99700.09.2073771
TTAGCCG42050.09.1045788
GAAAAAT159750.08.9867351
CGCGAAT31550.08.9694121
GGCGAAT39950.08.897541
GGGGAAT75700.08.8897221
GGAAAAC92450.08.8842241
TAGCGTC61350.08.7703169
GGGAAAC86600.08.7272161
GTTAGCG60550.08.6013467
TAGGTAC55950.08.56875210
Produced by FastQC (version 0.11.2)