FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_z1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_z1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27817472
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT136650.011.0099011
GGGAAAT88600.010.0482991
CGCGTAT21850.09.4755921
TTAGCGT51150.09.4401848
TAGCGTC51600.09.4247159
CCGGGAT119500.09.1537261
GTTAGCG51450.09.1170747
CGGAAAT42050.08.9447261
CGGGCAT67550.08.735291
ATACCTA46650.08.7251035
GGAAAAC93350.08.6868051
GGGAGAT141150.08.4097691
CGGGGAT104050.08.3904441
GGAAGAT254500.08.3250551
ACCTAGG47300.08.3127687
GGCGAAT39000.08.22857951
GGGGAAT73600.08.2047771
TACCTAG53300.08.0887549
CGGAGAT255000.08.0380891
CCGGAAT56050.08.0033951
Produced by FastQC (version 0.11.2)