FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_y5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_y5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30871123
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG57900.012.3904027
ATACCTA63450.011.3619055
CGCGTAT31900.010.7092321
CGCGAAT42550.010.7050371
CCTAGGT70500.010.6161848
TACCTAG67500.010.5269216
TAAGTCG27700.010.3346419
TAGGTAC70850.010.17432610
GGAAAAT160700.09.9206461
CGGGACT37950.09.7293941
CGGGGAT106900.09.5872171
GTGGATA85600.09.2718821
GGAAGAT265800.09.1916211
CTAGGTA80950.08.9474569
GTCCCTA37450.08.9334198
GTCGAAT51850.08.8514971
GCGAATC60900.08.8358582
GGGAAAC103500.08.76857951
CGGGCAT68900.08.7145271
CGGATAT45150.08.7128641
Produced by FastQC (version 0.11.2)