FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_y4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_y4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30108988
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT145200.011.621941
CGCGAAT39850.011.1711461
ACCTAGG54850.09.872227
CGGAAAT46900.09.7126441
ATACCTA58400.09.5674018
CCTAGGT61750.09.5510468
CGCAAAT49300.09.3098161
GGGAAAT93450.09.2689561
TTAGCCG42200.09.2354448
CGACAAT81200.09.1798181
GCCGAAT45500.09.0255141
CCGGGAT129650.09.0232621
TAGCGTC59550.08.9773079
CGGCTAT37500.08.9264111
GGCGAAT39950.08.897271
TTAGCGT60100.08.8950638
GGGAAAC93400.08.8674911
CGCGTAT23550.08.7921831
TACCTAG65650.08.7736019
CGCGATA23050.08.6834731
Produced by FastQC (version 0.11.2)