FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_y3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_y3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28958987
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG50800.013.98656510
ATACCTA54750.013.0403738
TCCCTAC54200.012.5364699
CCTAGGT56950.012.41557811
TACCTAG61650.012.3082959
TTCCCTA59150.011.4289188
CTACACG57900.011.37786712
CCCTACA69050.010.98921210
GGGAAAT98700.010.7338811
TAGGTAC67450.010.5339713
GGAAAAT149600.010.2881871
CTAGGTA69600.010.15899412
GCGGAAT36400.010.1442041
GCCGAAT47150.010.0270791
TACACGA65200.09.94528213
GGCGAAT45400.09.5014471
CCGGGAT117600.09.3315841
TTAGCCG44400.09.2441678
GATACCT82200.09.1891987
CGGGTAT23650.09.04682351
Produced by FastQC (version 0.11.2)