FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_y2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_y2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28998629
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT141900.010.7249481
CCGGGAT117950.09.3917411
CGACAAT78150.09.3173511
TACCTAG55250.09.2391779
CGGGCAT73950.09.1932081
ATACCTA52150.09.1931658
ACCTAGG49400.09.14632610
GGGGAAT72800.08.9592051
GGGAAAT91150.08.8971361
CGGAAAT42750.08.8796441
CGGGACT35800.08.77197551
GGAAAAC96950.08.4716541
TTAGCGT45550.08.3293078
CGGGGAT110750.08.2573591
GCCGAAT43500.08.1712221
GTTAGCG46550.08.1504037
CGGGTAT25150.08.09567451
CGGAAAC53750.08.0896761
GGAGAAT110300.08.0094641
GGCGAAT45700.08.0043981
Produced by FastQC (version 0.11.2)