FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_y1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_y1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28577122
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT98400.09.8544251
GGAAAAT159100.09.7819731
GCCGAAT45900.09.3976111
CCTAGGT47500.08.8587718
ACCTAGG43750.08.8296657
CGGAAAT49600.08.6965791
GGAAGAT262550.08.6615081
CGCGAAT31350.08.585731
GGAAAAC109900.08.5406581
CGGGTAT26350.08.5124161
CGCGTAT20700.08.5019781
CGGGCAT66300.08.2756811
GAAAAAT185150.08.1820281
GGGAGAT144200.08.0407051
CGGGGAT100800.08.0107921
CGGCAAT90950.08.0057111
GGGAAAC86150.07.97109371
CTCGGAT65350.07.920741
CCGGAAT60050.07.8727721
CGAAAAT60600.07.8582631
Produced by FastQC (version 0.11.2)