FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_x5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_x5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29292546
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT133600.011.8817561
ACCTAGG48250.011.723392510
GGGAAAT83800.011.448011
ATACCTA53700.011.36858758
TTAGCCG43700.010.7340848
CGGGTAT24350.010.6289791
CCTAGGT52800.010.51705811
CGGCTAT42050.010.4223811
GCCGAAT40900.010.2935641
TACCTAG60300.010.1242829
CCGGGAT141450.010.0025361
GGCGAAT35850.09.818391
TTAGCGT52250.09.7037278
CGGGCAT71200.09.6450011
GAAAAAT176000.09.5879431
CGGGGAT129000.09.55009651
TAGCGTC54750.09.32364759
CGCGTAT20500.09.2584461
GTTAGCG54950.08.9758717
AAAATCC127450.08.7687843
Produced by FastQC (version 0.11.2)