FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_x4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_x4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30372007
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG44250.010.6785338
GGAAAAT153950.010.5353881
GGGAAAT96700.09.6710851
GGGAAAC91050.08.9446731
CCGGGAT135200.08.9335431
CGGGGAT121350.08.8725241
GGAAAAC98750.08.8412931
AATTTAG76900.08.7915945
CGCAAAT47050.08.6547551
GGCGAAT41900.08.64784051
CGGAAAT46000.08.5522311
GGGGAAT74750.08.5406891
GTGAAAT136950.08.5170091
GTCGGAT65600.08.4694351
GGAAGAT277450.08.3831571
GGACTAA44050.08.30438362
CGCGTAT22650.08.2273121
GGCAAAT111200.08.2238181
GCCGAAT50550.08.1920481
GTTTAAT78400.08.1870841
Produced by FastQC (version 0.11.2)