FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_x3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_x3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28330065
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG46950.010.3582658
GGAAAAT139500.010.1916981
GGGAAAT88850.09.9039011
TTAGCGT48150.09.1688988
CGCAAAT44550.09.14024451
ACCTAGG45100.09.1008097
GTCGAAT52600.08.92229751
CGCGAAT36900.08.884251
GGGAAAC90300.08.7512731
GGGAGAT142000.08.6513761
GAAAAAT168150.08.6399011
GGCGAAT38950.08.593851
GTTAGCG49800.08.5881967
TAGCGTC53050.08.5820859
GGGGAAT75200.08.5811851
GGAAAAC89100.08.5592971
AATTTAG70450.08.5195415
GGAAGAT262250.08.5135821
CCGGGAT123900.08.4947781
GTGAAAT149050.08.3810831
Produced by FastQC (version 0.11.2)