FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_x2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_x2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32578378
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT176700.010.2923461
CGCGTAT23500.010.1326141
GGGAAAT110350.09.10040951
CTCGAAT61100.09.0368911
GTCGAAT57900.08.8211151
CGGGGAT110100.08.776291
CCGGGAT125200.08.5998441
GGAAGAT305050.08.5977111
GGGGAAT85750.08.4513371
GGGAAAC101800.08.3731611
GGAAAAC116800.08.2728561
CGGGCAT76850.08.2625531
GTCGGAT66950.08.247261
CGCGAAT35950.08.0634451
GGGAGAT170850.07.9785211
GGCGAAT48900.07.9746181
GGCAAAT127050.07.87705041
CCCGTAT24250.07.68461851
GCCAGAT142400.07.6338011
GGAAATT140600.07.58426431
Produced by FastQC (version 0.11.2)