FastQCFastQC Report
Sun 15 May 2016
HNC57BGXX_n01_js_dosematrix_x1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC57BGXX_n01_js_dosematrix_x1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35664268
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT187900.011.0749621
GGGAAAT121850.010.6208051
CGCGAAT44950.09.7504731
GTCGAAT68850.09.4233561
CCCGAAT40900.09.2815621
CCGGGAT137100.09.1625341
CGCGTAT25400.08.8314361
GGGAGAT180250.08.7305431
CGGAAAT57550.08.6950961
CGGCTAT50500.08.6105491
CTCGGAT83950.08.5505551
GGAAGAT331850.08.4963411
GCCGAAT57850.08.4710391
CGGGGAT122750.08.4343461
CGGGCAT85250.08.258241
GGGGAAT98850.08.2392371
GCGAATC70650.08.1534872
CTCGAAT64200.08.1169631
CTGGAAT179300.07.98765851
GGAAAAC122550.07.9693851
Produced by FastQC (version 0.11.2)