FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_3-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_3-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741295
Sequences flagged as poor quality0
Sequence length6
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGA25636434.58326307340533No Hit
TCGTGT471716.363323643084062No Hit
CGCGCG402735.432789914946142No Hit
TCTTGA389595.25553254777113No Hit
GGGGGG389595.25553254777113No Hit
GTAACG370725.000978018197883No Hit
CTAACG324384.375855765923148No Hit
GGCGCG147901.995157123682205No Hit
TCGGGT136341.8392138082679637No Hit
ACCTCT129151.7422213828502824No Hit
TCGTGA112061.5116788862733461No Hit
TCGGGA108571.4645991137131642No Hit
TCCTCT98101.3233597960326187No Hit
CCCTCT60250.8127668472065777No Hit
ATCATG52050.7021496165494169No Hit
CTACCG51370.6929764803485792No Hit
TCAGGA49770.6713926304642551No Hit
TCAATA43010.580200864702986No Hit
GTACCG39100.527455331548169No Hit
CTACTT37340.5037130966754126No Hit
TCGTCT34410.46418767157474417No Hit
GGACCG33220.44813468322327815No Hit
GCTGGA32490.43828705171355536No Hit
GGAACG29410.39673814068623153No Hit
AAACTA29180.39363546226535995No Hit
TCTGTA29060.39201667352403563No Hit
TCAGCA28470.38405762887919115No Hit
GGCCCG27090.36544155835396164No Hit
GGAGCG25420.3429134150371984No Hit
CGCGCT24900.3358986638247931No Hit
TCTTTA23090.3114819336431515No Hit
TCACTA23010.3104027411489353No Hit
TCTCGA22670.30581617304851644No Hit
TCTTGT21610.2915168725001518No Hit
TAAACA21580.2911121753148207No Hit
GGCTCG21160.2854464147201856No Hit
CCCGCT18350.2475397783608415No Hit
CGCTCG18290.24673038399017935No Hit
TCACGA18190.2453813933724091No Hit
GTAACT17690.23863644028355785No Hit
AAAATA17220.23229618438003766No Hit
GGGGCG16970.22892370783561203No Hit
TTAATA16170.21813178289345No Hit
TCTGGT15060.20315798703620017No Hit
GCCTCT13870.18710499868473415No Hit
TCTCTA13800.18616070525229497No Hit
TAACTA13790.18602580619051795No Hit
TCTATA13770.1857560080669639No Hit
TCAAGA13470.18170903621365314No Hit
CTCACG13180.1777969634221194No Hit
ACAGCA13030.17577347749546401No Hit
TAAATA12930.17442448687769377No Hit
TCATGA11750.15850639758800478No Hit
CGCTCT11740.15837149852622776No Hit
TCTGCA11640.1570225079084575No Hit
TCATTA11400.1537849304258089No Hit
GTAACA11340.15297553605514674No Hit
CGAGCG11150.15041245388138327No Hit
CGAACG11150.15041245388138327No Hit
GGATCG11040.14892856420183598No Hit
TCAGTA10610.14312790454542387No Hit
TAACGA10390.14016012518632934No Hit
CGACCG10050.13557355708591046No Hit
GGCACG9850.13287557585036996No Hit
TATGGA9340.12599572369974166No Hit
TAAAGA9160.12356754058775521No Hit
CGCCCG8970.12100445841399174No Hit
GGTGGG8570.11560849594291073No Hit
ACCGCT8210.11075212971893782No Hit
CCAGCA8170.11021253347182969No Hit
GTACCA8150.10994273534827566No Hit
TCTAGA7920.10684005692740406No Hit
TCCTGT7780.10495147006252571No Hit
GTCACG7610.1026581860123163No Hit
GCGTCT7540.1017138925798771No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)