FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_3-1-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_3-1-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4331568
Sequences flagged as poor quality0
Sequence length6
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATG232630153.705748126313615No Hit
ATCATT2904526.705470166923387No Hit
CTCATG1594903.6820384673633195No Hit
ATAATG1542093.5601195687104528No Hit
TTCATG1450053.3476330049534027No Hit
GTAACG1158682.6749666633422353No Hit
TCGTGT1036962.393959877808683No Hit
GGGGGG672471.5524863051901758No Hit
TTCATT598651.3820630312164093No Hit
CTACTT565461.3054395082796808No Hit
CGCGCG517741.1952715506255471No Hit
CTAATG466581.0771618961078298No Hit
ATCAAG457041.0551375391082398No Hit
ATAAAG439361.0143209110419138No Hit
TTAATG364320.8410811050409459No Hit
AACATT351750.8120615906295364No Hit
TCTGGA333470.7698597828777015No Hit
ATAATT323840.7476276489252852No Hit
GTCATG259670.5994826815601186No Hit
CTCAAG259010.597958983906059No Hit
CTAAAG227630.5255140863539485No Hit
TGCATG206230.47610934423746776No Hit
GTAATG156480.3612548619806962No Hit
CTAACG135710.3133045585339997No Hit
CTAATT129390.29871399917997365No Hit
TCCATT120800.2788828433491059No Hit
TGGTTG120280.27768235428833166No Hit
ACCATT119010.2747503906206713No Hit
ATAATA105270.24302977582251972No Hit
TGGTGG95250.21989727507452267No Hit
TTGATT89560.2067611543902808No Hit
TTAAAG89360.20629942782844457No Hit
AGCATG86620.1999737739312877No Hit
CTCACG85400.19715724190408646No Hit
TGCATT73880.17056179194231744No Hit
TGGATG70900.16368206617095704No Hit
AAAATT68780.1587877646154926No Hit
ATAACG68490.15811826110083No Hit
TCGATT68340.1577719661794528No Hit
TTAACG61850.1427889392478659No Hit
TCGTTT59190.13664797597544354No Hit
TTAATA57250.13216922832563172No Hit
GTCACG57180.13200762402898902No Hit
GTAACT54400.12558962481946492No Hit
TTGATG54340.125451106850914No Hit
ATCATA53420.1233271646664672No Hit
GTAAAG50570.11674756116030038No Hit
AACATG49480.11423115139829272No Hit
AAAATA48920.11293831702515117No Hit
TAAACT45700.10550451937958724No Hit
CTAAAT45030.10395773539743575No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)