FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_3-1-1_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_3-1-1_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884906
Sequences flagged as poor quality0
Sequence length6
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACG95499733.10322762682736No Hit
CTAACG35187612.197139178884859No Hit
TAAACA1353574.69190330638156No Hit
GTAACT1317614.56725453099685No Hit
GTAACA1091843.7846640410467445No Hit
CGCGCG1037703.596997614480333No Hit
TCCTCT649402.2510265499118516No Hit
GGAACG579722.009493550223127No Hit
TCTGGA577442.0015903464445635No Hit
TAAACT493751.7114942393270351No Hit
TCGTGT492351.7066413949016017No Hit
ACCTCT422631.4649697425150074No Hit
GGCGCG399111.3834419561677227No Hit
GTACCG345491.197578014673615No Hit
GGGGGG332841.1537290989723754No Hit
GGCTCG244360.8470293312849708No Hit
GGACCG233410.8090731552431865No Hit
CCCTCT193840.6719109738757519No Hit
TCGTCT185020.6413380539955201No Hit
GTCACG174540.6050110471537027No Hit
GGCACG167640.5810934567712085No Hit
GGCCCG157780.5469155667463689No Hit
GTAACC152330.5280241366616452No Hit
GTACCA145800.505389083734444No Hit
ATCATG144750.5017494504153688No Hit
CTACCG144510.5009175342281516No Hit
TCAACA132500.4592870616928247No Hit
GCAACA121440.420949590731899No Hit
GCCTCT111580.3867717007070594No Hit
GGATCG105480.3656271642819558No Hit
TCAACT101250.35096464148225276No Hit
TTAACA101150.35061800973757895No Hit
TCAGCA100230.3474289976865797No Hit
TTAACG100140.34711702911637327No Hit
TTAACT96420.3342223282145068No Hit
GGAGCG91420.3168907409808153No Hit
CTCACG87510.3033374397640686No Hit
GTACCT86180.2987272375599066No Hit
CGCTCG83800.29047740203666944No Hit
CGAACG66820.23161933179105315No Hit
GGAACA64660.22413208610609844No Hit
ACAGCA62090.21522365026798101No Hit
GGAACT61090.2117573328212427No Hit
GAAACA58000.20104641191082137No Hit
ATCAAG55420.19210331289823657No Hit
TCTTGA54650.18943424846424806No Hit
CTACTT51670.17910462247296793No Hit
GCAACT43960.15237931495861565No Hit
GCGTCT43320.15016087179270313No Hit
CCAGCA41850.14506538514599782No Hit
TCCACT40270.1395886035801513No Hit
GGGGCG39710.13764746580997786No Hit
CGCTCT38060.13192804202285965No Hit
TCCTGT37830.13113078901010985No Hit
GGCTCT35600.12340090110388345No Hit
GCACCA32690.11331391733387501No Hit
TAAAGT31900.11057552655095175No Hit
CGCGCT31070.10769848307015896No Hit
TAACCA30980.10738651449995253No Hit
CTAACT30520.10579200847445289No Hit
CGACCG29010.10055786912987806No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)