FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_2-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_2-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190706
Sequences flagged as poor quality0
Sequence length6
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACG6972136.55941606451816No Hit
GGGGGG6152132.25960378802974No Hit
ATCATG59303.109498390192233No Hit
GTCACG45242.372237894979707No Hit
CTAACG36661.9223307080007972No Hit
TTAACG25191.3208813566432098No Hit
ATAAAG25151.3187838872400448No Hit
TCTGGA24541.2867974788417775No Hit
CTAAAG23861.2511404989879709No Hit
CGCGCG21011.101695804012459No Hit
GTAAAG15860.8316466183549547No Hit
CTACTT15810.8290247816009985No Hit
GTACCG15320.8033307814122261No Hit
GTCATG15300.8022820467106437No Hit
GTAACT15250.7996602099566872No Hit
GTAATG14930.7828804547313667No Hit
TTAAAG14480.7592839239457594No Hit
CTCATG13000.6816775560286514No Hit
TTAATG10940.5736578817656497No Hit
TCGTGT10390.54481767747213No Hit
CTCACG9980.5233186160896878No Hit
TTCATG9930.5206967793357314No Hit
ATAACG9630.5049657588119932No Hit
CTCAAG8910.4672113095550219No Hit
ATAATG8890.4661625748534393No Hit
CTAATG7510.393799880444244No Hit
TTCACG7110.3728251864125932No Hit
ATCAAG6200.3251077574905876No Hit
CTAACT5960.3125229410715971No Hit
GTCAGG4930.25851310394009624No Hit
GGAACG4470.23439220580369785No Hit
CTAAAT4300.22547796084024624No Hit
GTAACA3530.1851016748293184No Hit
GTACCT2660.1394817153104779No Hit
GTAAGG2530.1326649397501914No Hit
ATCACG2380.12479942948832234No Hit
CTACCG2200.11536081717407948No Hit
GGACCG1970.10330036810588025No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)