FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_2-1-2_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_2-1-2_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547242
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGT47130830.461168970335606No Hit
CTACTT18029411.65260508698704No Hit
ATCATG16930010.94205043554919No Hit
GTAACG1123707.262600162094876No Hit
GGGGGG877005.668150166554424No Hit
TCTGGA407682.6348819383134634No Hit
CGCGCG279751.8080558826608897No Hit
TGGTGG249461.6122881876267579No Hit
TCGTGG218301.410897584217595No Hit
TTCATG174501.1278132315436111No Hit
TCCTGT130960.8464092882690619No Hit
TCGTTT127140.8217201963235228No Hit
TCCTCT120710.78016237925289No Hit
ACCTCT100860.6518695847191325No Hit
TCCATT90280.5834898483882935No Hit
GTCATG84340.5450989567242875No Hit
TCGAGT79730.515304005449697No Hit
TAAACT79220.5120078177815752No Hit
TTCATT76300.49313552760330964No Hit
TCTTGT73120.4725828280256094No Hit
CTCATG71860.4644393055514263No Hit
TCCAGT70390.4549385293315461No Hit
TCTTGA69820.4512545548789394No Hit
TGGTTG68850.44498533519643335No Hit
GTAATG64230.41512575279109537No Hit
TCGATT63720.4118295651229737No Hit
GTAACT59810.38655879300070706No Hit
TTAATG59020.3814529336716557No Hit
ATCATT57030.3685913386529063No Hit
CTAATT52880.34176941939270006No Hit
TAAATT49810.3219276622532222No Hit
AAAATT46580.3010518070217846No Hit
GTCACG43950.28405381963519605No Hit
TAAAGT43470.2809515253593168No Hit
TCGTCT42640.27558714150727553No Hit
ATAATG39750.25690874472125236No Hit
ACCATT38140.24650313267090734No Hit
AACATT37680.243530100656523No Hit
GTCAGG36740.23745477436625945No Hit
GCGTGT35440.22905272736908644No Hit
TTAACG34740.2245285482167625No Hit
ACCTGT32400.209404863621851No Hit
TCGGGT31550.2039112175083148No Hit
TCAACT30380.19634937521085905No Hit
CTAATG29480.19053257344358543No Hit
TCAAGT27770.17948065008576553No Hit
TCGTGA27510.1778002406863309No Hit
TGGTGT26500.17127249648083492No Hit
TTCAGG26260.1697213493428953No Hit
CCCTCT25580.16532643245206632No Hit
GTACTT25100.16222413817618705No Hit
TCCTTT24430.15789385241610557No Hit
TACATT23940.15472692700947882No Hit
TTGATT23440.15149537047210454No Hit
TGCATG22930.14819918280398284No Hit
GTAAGT20990.13566074343897078No Hit
GTAATT20430.13204140011711163No Hit
GTAAGG20180.1304256218484245No Hit
GGGGGT19970.12906836810272732No Hit
TCGTTG19750.12764648322628264No Hit
CTAACG19420.12551365591161565No Hit
ACCTTT19220.12422103329666594No Hit
ATAATT18990.1227345172894738No Hit
TCATGT18130.11717624004519009No Hit
TTCAGT18110.11704697778369512No Hit
TGGATG17990.11627140421472529No Hit
TCCACT16030.10360370258821826No Hit
TACAGT15590.10075993283532893No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)