FastQCFastQC Report
Sun 22 Dec 2024
HNC3VDRX5_n02_Rep_2-1-1_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_n02_Rep_2-1-1_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434573
Sequences flagged as poor quality0
Sequence length6
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCG94225627.434443815868814No Hit
ATCATG35423110.313683826199064No Hit
CTACTT1735305.0524475677180245No Hit
CCAGCA1257753.66202727384161No Hit
ACAGCA1144323.3317678791512075No Hit
GGGGGG1089713.1727670368339815No Hit
ATCAAG824392.400269262001419No Hit
TCTGGA815182.3734537015227217No Hit
CTCATG717242.0882945274419846No Hit
GTAACG607881.7698852229956972No Hit
ATAATG579241.6864978557742112No Hit
CGAGCA497321.4479820344479504No Hit
CTCAAG467861.3622071797571345No Hit
CTACAT431221.255527251859256No Hit
TCAGCA366491.0670613202863937No Hit
CGCGTG323470.9418055752490921No Hit
TTCATG311020.905556527696456No Hit
CGAGCG281310.8190537804845027No Hit
CGCCCG269020.7832705841453945No Hit
CTCCAG267530.7789323447194163No Hit
CGCGCT266730.7766030886517771No Hit
CTAATG257040.7483899745324964No Hit
TTAATG251370.7318813721531031No Hit
CGAGCT249650.7268734716076787No Hit
ATAAAG231740.6747272513934046No Hit
CGCTCG228420.6650608387127017No Hit
CTAACG214740.6252305599560702No Hit
CGCGGG204930.5966680574266437No Hit
CGCCTG187410.5456573495453438No Hit
TCGTGT182760.5321185486521905No Hit
ATCATT181080.5272271109101481No Hit
AAACTA170900.49758732744943845No Hit
GTCATG170100.49525807138179917No Hit
CTCCCG157820.45950399074353643No Hit
CTCCTG155210.4519047928228633No Hit
ATAATA154860.45088574329327114No Hit
CTCACG149250.4345518351189508No Hit
GTAATG144910.42191562095200774No Hit
CTAAAG136310.3968761182248856No Hit
GCAGCA125950.3667122521489571No Hit
ATAATT120630.35122269929915595No Hit
CTCGCG112430.3273478246058535No Hit
CGCATG105560.30734533812500126No Hit
CGCGCA105280.3065300985013275No Hit
CGCTTG103640.30175512356266704No Hit
TTAATA97920.2851009426790463No Hit
CTAATT90820.2644287950787478No Hit
CTACCT83380.2427667136497026No Hit
ATCCAG81670.23778792880512367No Hit
AAAATA77910.2268404252872191No Hit
CGAGCC76940.2240162023052065No Hit
CTACCG76330.22224014455363156No Hit
CTACTA75510.21985265708430132No Hit
GTCACG70830.20622650908861162No Hit
TTAACG70540.20538215376409236No Hit
AGCGCG68540.1995590135949942No Hit
CGCCAG68510.19947166649245773No Hit
CCCGCA68400.19915139378315733No Hit
TCAATA66470.1935320635199776No Hit
TTAAAG65300.19012552652105516No Hit
ATCACG61060.17778046936256706No Hit
GGAGCA60780.1769652297388933No Hit
AGCATG54590.1589426109155345No Hit
TCAGGA52520.15291566084051786No Hit
CGCGAG50380.14668490085958283No Hit
CGCGCC49850.14514176871477183No Hit
GGCGCG49790.14496707450969887No Hit
AGCTCG48600.14150230610908546No Hit
AGCAAG47870.13937685994736465No Hit
CGCTAG45190.1315738521207731No Hit
CTAAAT44480.12950663736074325No Hit
ATAACG43870.1277305796091683No Hit
ATCCTG43100.12548867064406552No Hit
CGCAAG42870.12481900952461922No Hit
ACCTCT42850.12476077812292824No Hit
TCACTA42180.12281002616628037No Hit
TCTGTA42100.12257710055951641No Hit
CGCACG40560.11809328262931083No Hit
GTAAAG39040.11366769610079623No Hit
TTCATT37070.10793190303423454No Hit
CCCTCT37000.10772809312831609No Hit
AGCTAG35800.1042342090268572No Hit
TCTTGA35260.1026619611812007No Hit
CCAGCT34360.10004154810510653No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)