FastQCFastQC Report
Mon 6 Jan 2025
HNC3VDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935936462
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA1151818025.949668507250833No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA969030575.0054874683175425No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA895731594.626864608328246No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG869946984.493675268150407No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC728254203.7617670532825573No Hit
GAGGCCAGAGGCCACTTGTGAAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA649422023.3545626767579315No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC625652923.23178437041081No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG625237853.229640343433957No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCC621922953.212517364115848No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG613987843.171528880476244No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT550449692.8433251855349373No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCT530790252.741775158527904No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC502233222.5942649971122864No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC493721562.550298368211632No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC475734312.4573859697261073No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA452690192.3383525176871225No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT388974512.0092317988481585No Hit
GAGGCCAGAGGCCACTTGTGAAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG352931871.8230550275156705No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC331919171.7145147917566315No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA326501241.686528697655161No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTC326101301.684462824069688No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC291472461.5055889783638985No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGC275801351.4246405055828737No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT255512781.3198407334920064No Hit
GAGGACAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA237265191.2255835594670461No Hit
GAGGACAGAGGCCACTTGTGAAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA134608230.6953132638502885No Hit
GAGGACAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG128598580.664270664478037No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGAC46493440.24015994797663975No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCC42484870.21945384486487346No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG37840210.1954620450761467No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG36711400.18963122354787282No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG36068880.18631231297094086No Hit
GAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAAA35780970.18482512573287127No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA34311250.17723334765105528No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG33196830.17147685707466157No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA32761080.16922600841018717No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC32221890.16644084468925097No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC29848540.15418140308780443No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA29448700.1521160460482096No Hit
ATGAGGCCAGAGGCCACTTGAGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA29265400.15116921745337736No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC27237490.14069413193375765No Hit
GAGGCCAGAGGCCACTTGTGAAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGAC27050830.13972994739741618No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA25727670.13289521895476342No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA25358090.13098616869792704No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCA24429150.12618776741651141No Hit
CGATGAGGCCAGAGGCCACTAGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG24232490.12517192829234494No Hit
GAGGCCAGAGGCCACTTGTGAAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCC23754890.12270490517782293No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT23284190.12027352372890016No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACT23130340.1194788178952125No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC22777770.11765763209226647No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC22357760.11548808774902862No Hit
GATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA21062640.10879819877063714No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTC20600150.10640922573831868No Hit
TCGATGAGGCCAGAGGCCACATGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA20599050.10640354373365793No Hit
GATGAGGCCAGAGGCCACTTCTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGA20571010.10625870426939663No Hit
TCGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGA20124310.10395129383125384No Hit
TGAGGCCAGAGGCCACTTGTCTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA20049870.10356677707948454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)