FastQCFastQC Report
Mon 6 Jan 2025
HNC3VDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3VDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935936462
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTT44306801122.886495486647846No Hit
CGCGCG38355122519.81218043714908No Hit
TCGTGT30190206115.594626524473199No Hit
GTAACG25189558913.011562824730763No Hit
ATCATG25129291012.980431689394981No Hit
TCTGGA22298742611.518323580187829No Hit
GGGGGG239085441.2349859858158918No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)