FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988113
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6044478.649645476539947No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1240081.7745563072606294Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89060.12744499123010747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA229750.064.111652
GCACACG110650.062.2546718
ATAACTG237900.061.9686161
ACTGAAG233650.061.355284
CACACGT81600.060.72232419
AGAGCAC159950.059.46031615
AGCACAC118300.058.03819317
ACACGTC53350.057.72361420
ATCGTCC37350.057.1354511
GTGACTG71150.057.0914041
GAGCACA153700.056.44845216
AACTGAA266800.056.323323
TGACTGG71500.055.9939652
CACGCGT13000.055.5954219
CTGAAGA288950.054.722725
GCACGCG18850.054.2892118
CGCGTCT11400.054.05515321
CACGTCT32500.053.3674421
TGAAGAT288250.052.61686
TCAGACG63950.052.39214712
Produced by FastQC (version 0.11.5)