FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720517
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29960.1741337051595538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC15100.037.5494045
CGGGCAA1900.035.0089071
TCGCGAG1251.05501385E-1030.8006782
CAAGATC15700.030.5411835
GCGAGAT6500.030.1536354
AAAAGGG52400.030.0570470
CCGTATC120350.028.8207455
CGTATCA122050.028.64872456
TCATTAA114900.028.05544760
CGCCGTA130100.028.03208453
CATTAAA120050.028.01729861
TCGAGAT6250.027.9998024
TCGCCGT130650.027.78013452
GCCGTAT133200.027.59069454
GTATCAT116250.027.4586757
TCCCGGA906.1656847E-627.2291551
GGTCGCC143350.026.5397550
GTCGCCG141900.026.51496351
CTAGATC11800.026.3981195
TGGTCGC142050.026.28985249
Produced by FastQC (version 0.11.5)