FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779175
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79400.1661374609634508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC24700.025.222585
CGGGCAA5600.023.755631
TGCGTCG8350.021.7965562
CTAGATC20400.021.7891375
CATTAAA217200.021.43178261
CCGTATC219950.021.05243555
AAAAGGG109000.020.87148570
TCATTAA217550.020.85030660
GTATCAT209700.020.82968157
CGTATCA224800.020.7227956
CAAGATC35400.020.56495
CGCGTAG3800.020.2632962
GTGCGTC4250.019.769391
CGCCGTA246300.019.69542353
ATCATTA230600.019.60964259
GCCGTAT250900.019.54357154
CGCGAAA8600.019.5395161
TCGCCGT249800.019.44748752
CGAGCAG11950.019.0421711
TATCATT235000.019.00418758
Produced by FastQC (version 0.11.5)