FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10271571
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167410.16298383178191533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG25850.023.5648441
CGAGATC28900.021.4358835
CCGTATC227300.021.08017555
CGCGGAG8750.020.8051641
CGTATCA233950.020.58569556
CGCCGTA245250.020.1793153
TCGCCGT248300.019.87505552
GCCGTAT255800.019.56616254
CGCGCAG6150.019.3543971
CGCGAAG16750.019.0196461
TGCGGTG17500.019.0047171
GTCGCCG270100.018.99657451
CGGGCAG10350.018.94191
GGTCGCC274650.018.8347950
TGGTCGC273450.018.62305849
GTATCAT242450.018.56474357
CGCGAAA18500.018.1666741
CATTAAA271850.017.84431361
GTGGTCG293000.017.58353248
GTCCGCA27300.017.30760813
Produced by FastQC (version 0.11.5)