FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405342
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159090.21483140143966342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC23900.060.3344543
TCGTGAC25850.057.4082532
GTGACCT27450.052.914154
TTCGTGA35450.046.01821
TCGCGAG10550.038.8152542
CGCGAGA18800.037.792383
CGAGATC115600.037.573365
GCGAGAT42650.035.6973764
AAAGGGG246500.034.8862570
CTAGATC91350.034.099465
TCGCGTA7400.034.061261
CGCGCAG4850.033.20291
AAAAGGG523550.033.18483770
CAAGATC145550.032.5110175
CGCTAGA21450.031.9812053
CGCGTAG8600.031.7442572
CCGAGAT25700.031.5951884
GCTAGAT56400.031.5867374
CGCGTGA4250.031.300741
CGTAGAT43550.031.0216714
Produced by FastQC (version 0.11.5)