FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437738
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63050.18340548348943403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG2650.030.3781222
CATTAAA168200.027.30072661
CGAGATC21050.027.2683015
CGCGAGA4800.026.9790613
TCATTAA164650.026.8690160
CCGTATC169250.026.71776855
CGTATCA170000.026.414656
GTATCAT162050.026.13380457
CGCGTAG2450.025.7149312
GCGAGAT10250.025.2681944
ATCATTA176350.024.90775559
TCGCGTA2250.024.8949451
CGCCGTA185900.024.8143253
GCCGTAT190300.024.71876754
AAAAGGG82850.024.7132470
CGCGGAC3000.024.49990349
TATCATT179150.024.3621758
TCGCCGT188700.024.26063352
CGGGCAG2950.023.7345891
CTCGCGA1202.1664473E-623.339011
Produced by FastQC (version 0.11.5)