FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689923
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1619961.2765719697432363Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1424451.1225048410459229No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56050.048.5813418
AGAGCAC99850.044.65672315
GAGCACA96300.044.5584116
AGCACAC73950.042.2174717
CACACGT45650.039.71499319
AAGGGGG273600.035.5754567
AAAGGGG290900.033.26725466
AGGGGGG317750.032.58202768
ACACTCT60800.030.5761681
TAGATCG213600.029.150116
CCGTATC570950.029.13635849
CAGATCG216150.028.8871786
CGTATCA581100.028.70573250
CGCCGTA581550.028.51500947
AAGAGCA173250.028.12104814
GCCGTAT595400.028.1103548
TCGCCGT588550.027.89041546
GTATCAT578650.027.79297651
GTCGCCG632700.026.89568545
ATCTCGG567750.026.8655436
Produced by FastQC (version 0.11.5)