FastQCFastQC Report
Thu 15 Aug 2019
HNC3GAFXY_n02_ncm3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNC3GAFXY_n02_ncm3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8692216
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG924341.0634112175767376No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC537820.6187375003106227Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31900.049.48249418
AGAGCAC59000.040.0421615
CACACGT29450.039.93210619
AGCACAC44750.039.26236317
GAGCACA58450.039.04170616
AAGGGGG109850.031.51098467
AAAGGGG120950.028.76379866
AGGGGGG132000.028.34454368
TAGATCG102500.026.9071736
CAGATCG106450.025.9087376
CCGTATC226600.025.26906249
CGCCGTA229550.025.2340247
GCCGTAT236450.024.66047348
TCGCCGT233050.024.65981346
CGTATCA233550.024.6070250
GATCGGA390100.024.4666738
GGTCGCC247650.024.16704244
ACACGTC19700.024.16244720
GAGATCG74650.024.1458856
AGATCGG396150.024.1106877
Produced by FastQC (version 0.11.5)